针对硫化氢阳性菌属在新陈代谢过程中释放出H2S的特性,设计了双面镀银的压电石英晶体探头,构建新型的生物传感器模式.信号探针修饰的Ag可与生物代谢产物H2S结合,通过压电石英晶体经典的气相质量响应的原理,实现了对硫化氢阳性菌属的检测.结果表明,该传感器能很好地区分硫化氢阳性、阴性菌属,培养基中最佳的半胱氨酸浓度为0.05％.该传感器的设计避免了培养基与探头之间的直接接触,减少了液相质量响应存在的干扰,成功实现了硫化氢阳性菌属的自动培养和检测.%A silver-modified piezoelectric quartz crystal probe was involved in constructing piezoelectric biosensor for detection of hydrogen sulfide positive genus which releases hydrogen sulfide during metabolism. This biosensor was based on the special affinity of hydrogen sulfide on silver and the classic piezoelectric quartz crystal gas-mass response. The results indicated that this biosensor showed an excellent specificity to distinguish the hydrogen sulfide positive genus from the negative. The optimum concentration of cysteine in culture medium was obtained to be 0. 05% . The biosensor avoided direct contact of the probe with culture medium, which could limited most influence in the liquid-mass response. This method would be expected to use in the automated detection of hydrogen sulfide positive genus during cultivation.
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