Molecular characterization of chalcone isomerase (CHI) regulating flower color in herbaceous peony (Paeonia lactiflora Pall.)

WU Yan-qing; ZHU Meng-yuan; JIANG Yu; ZHAO Da-qiu; TAO Jun

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Molecular characterization of chalcone isomerase (CHI) regulating flower color in herbaceous peony (Paeonia lactiflora Pall.)

机译：查尔酮异构酶（CHI）调节草本牡丹（color药牡丹花）中花色的分子表征。

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Chalcone isomerase (CHI) is a key enzyme that converts yellow chalcone to colorless naringenin, playing an important regulatory role in color formation of ornamental flowers. We determined the coding sequence of CHI in herbaceous peony using rapid-amplification of cDNA ends (RACE) technology, and subsequently detected the expression pattern of CHI in the inner and outer petals at different developmental stages using qRT-PCR. We cloned the upstream promoter sequences of CHI using genome walking technology and predicted the location of CpG islands and 5′ truncation. In addition, we constructed five dual-luciferase reporter gene carriers and detected the promoter activities of different fragments. Our results showed that the full-length cDNA sequence of CHI was 898 bp, and the 5′-upstream core promoter was located at –1651 to –2050 bp region, where contained one CpG island (–1897 to –2010 bp) and several important binding sites of transcription factor, such as Sp1, serum response factor (SRF), activating protein (AP)-2alpha and CCAAT/enhancer binding protein (C/EBP)alpha. Expression results showed that the expression of CHI at different developmental stages was generally higher in inner petals than those in outer petals, and the maximum at the bud stage (S1). Thus, this study will provide theoretical basis for an in-depth study of CHI gene function and expression regulation.

机译：Chalcone异构酶（Chi）是一种关键酶，将黄色醌转化为无色鸣曲素，在观赏花的颜色形成中发挥着重要的调节作用。我们确定使用CDNA末端（RACE）技术的快速扩增的草本牡丹中CHI的编码序列，随后使用QRT-PCR检测不同发育阶段的内部和外花瓣中CHI的表达模式。我们使用基因组步行技术克隆了CHI的上游启动子序列，并预测了CPG岛和5'截断的位置。此外，我们构建了五种双荧光素酶报告基因载体，并检测了不同碎片的启动子活性。我们的研究结果表明，志法的全长cDNA序列为898磅，5'-上游核心启动子位于-1651至-2050 bp区，其中包含一个CpG岛（-1897至-2010bp）和几个转录因子的重要结合位点，例如SP1，血清响应因子（SRF），活化蛋白（AP）-2Alpha和CCAat / Enhancer结合蛋白（C / EBP）α。表达结果表明，在不同发育阶段的CHI表达通常在内部花瓣中的比外花瓣中的表达更高，并且在芽阶段的最大值（S1）。因此，该研究将为CHI基因功能和表达调节的深入研究提供理论依据。

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《农业科学学报（英文版）》 |2018年第1期|122-129|共8页
作者
WU Yan-qing; ZHU Meng-yuan; JIANG Yu; ZHAO Da-qiu; TAO Jun;
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作者单位

Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, P.R.China;

Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, P.R.China;

Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, P.R.China;

Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, P.R.China;

Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, P.R.China;

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收录信息中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
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正文语种 eng
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Molecular characterization of chalcone isomerase (CHI) regulating flower color in herbaceous peony (Paeonia lactiflora Pall.)

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