Identification of the strain-specifically truncated nonstructural protein 10 of porcine reproductive and respiratory syndrome virus in infected cells

ZHANG Zhi-bang; XU Lei; WEN Xue-xia; DONG Jian-guo; ZHOU Lei; GE Xin-na; YANG Han-chun; GUO Xin

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Identification of the strain-specifically truncated nonstructural protein 10 of porcine reproductive and respiratory syndrome virus in infected cells

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The nonstructural protein 10 (nsp10) of porcine reproductive and respiratory syndrome virus (PRRSV) encodes for helicase which plays a vital role in viral replication. In the present study, a truncated form of nsp10, termed nsp10a, was found in PRRSV-infected cells and the production of nsp10a was strain-specific. Mass spectrometric analysis and deletion mutagenesis indicated that nsp10a may be short of about 70 amino acids in the N terminus of nsp10. Further studies by rescuing recombinant viruses showed that the Glu-69 in nsp10 was the key amino acid for nsp10a production. Finally, we demonstrated that nsp10a exerted little influence on the growth kinetics of PRRSV in vitro.

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《农业科学学报（英文版）》 |2018年第5期|1171-1180|共10页
作者
ZHANG Zhi-bang; XU Lei; WEN Xue-xia; DONG Jian-guo; ZHOU Lei; GE Xin-na; YANG Han-chun; GUO Xin;
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Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University/State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.China;

Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University/State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.China;

Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University/State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.China;

Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University/State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.China;

Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University/State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.China;

Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University/State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.China;

Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University/State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.China;

Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University/State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.China;

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Identification of the strain-specifically truncated nonstructural protein 10 of porcine reproductive and respiratory syndrome virus in infected cells

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