Evaluation of parameters affecting Agrobacterium-mediated transient expression in citrus

LI Fang; DAI Su-ming; DENG Zi-niu; LI Da-zhi; LONG Gui-you; LI Na; LI Yi; Alexandra Gentile

首页> 中文期刊> 《农业科学学报（英文版）》 >Evaluation of parameters affecting Agrobacterium-mediated transient expression in citrus

Evaluation of parameters affecting Agrobacterium-mediated transient expression in citrus

开具论文收录证明 >>

期刊封面封底目录下载 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Agrobacterium-mediated transient expression assays are a convenient alternative to stable expression because they are simple,easy to perform,and achieve gene expression rapidly.This study investigated the factors affecting transient gene expression efficiency in citrus by observing the cryo-sectioning of leaf samples under a laser confocal microscope.These factors included the composition of the infiltration buffer,the Agrobacterium cell density,the leaf development stage,the incubation temperature,and plant genotype.The highest transient expression level of yellow fluorescent protein (YFP) was detected in Mexican lime (Citrus aurantifolia) on the third day after the intermediate-aged leaves were infiltrated with the improved infiltration buffer 1 (15 mmol L-1 2-(N-morpholino)ethanesulfonic acid,10 mmol L-1 MgCl2,and 200 μmol L-1 acetosyringone),which had an optical density of 0.8 and was incubated at 22℃.Additionally,this transient expression assay was applied to other citrus genotypes.Of note,trifoliate orange (Poncirus trifoliata) and kumquat (Fortunella obovate) had higher expression efficiency than other six genotypes of the Citrus genus.Our study provides research basis for the selection of optimization strategies in transient gene expression and improves the method for available genome investigation in citrus.

著录项

来源
《农业科学学报（英文版）》 |2017年第3期|572-579|共8页
作者
LI Fang; DAI Su-ming; DENG Zi-niu; LI Da-zhi; LONG Gui-you; LI Na; LI Yi; Alexandra Gentile;
展开▼
作者单位

National Center for Citrus Improvement Changsha/Horticulture and Landscape College,Hunan Agricultural University,Changsha 410128,P.R.China;

National Center for Citrus Improvement Changsha/Horticulture and Landscape College,Hunan Agricultural University,Changsha 410128,P.R.China;

National Center for Citrus Improvement Changsha/Horticulture and Landscape College,Hunan Agricultural University,Changsha 410128,P.R.China;

National Center for Citrus Improvement Changsha/Horticulture and Landscape College,Hunan Agricultural University,Changsha 410128,P.R.China;

National Center for Citrus Improvement Changsha/Horticulture and Landscape College,Hunan Agricultural University,Changsha 410128,P.R.China;

National Center for Citrus Improvement Changsha/Horticulture and Landscape College,Hunan Agricultural University,Changsha 410128,P.R.China;

National Center for Citrus Improvement Changsha/Horticulture and Landscape College,Hunan Agricultural University,Changsha 410128,P.R.China;

Department of Plant Science,University of Connecticut,Connecticut 06269,USA;

National Center for Citrus Improvement Changsha/Horticulture and Landscape College,Hunan Agricultural University,Changsha 410128,P.R.China;

Department of Agriculture and Food Science,University of Catania,Catania 95123,Italy;

展开▼
原文格式 PDF
正文语种 eng
中图分类
关键词

Evaluation of parameters affecting Agrobacterium-mediated transient expression in citrus

摘要

著录项

相关主题

期刊订阅