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Role of duck plague virus glycoprotein C in viral adsorption: Absence of specific interactions with cell surface heparan sulfate

机译：鸭瘟病毒糖蛋白C在病毒吸附中的作用：与细胞表面硫酸乙酰肝素缺乏特异性相互作用

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摘要

Many mammalian herpes viruses utilize heparan sulfate (HS) moieties present on cell surface proteoglycans as receptors for cellentry, and this process also requires viral glycoprotein C (gC) homologues. However, our understanding of the role of gC in facilitating attachment of other alpha-herpes viruses such as the duck plague virus (DPV) remains preliminary. To study the role of gC during DPV infection, we used a gC-deleted mutant virus (DPV-ΔgC-EGFP). Examination of the viral copy number by real-time PCR, as well as time course studies of viral adsorption and proliferation revealed that gC was involved in the viral binding to the cell surface. The affinity of viral glycoproteins (gB-DPV, gC-DPV, and gE-DPV) to HS was assessed using a prokaryotic expression system and HiTrapTM Heparin HP column chromatography. In addition, to confirm that gC played a role in the interaction between DPV and HS, viruses were treated with the HS analogue heparin and host cells were treated with its inhibitors heparinase prior to exposure to DPV-ΔgC-EGFP or wild-type strain Chinese virulent duck plague virus (DPV-CHv). The effects of heparin and heparinase on virus infectivity demonstrated that function of gC on viral adsorption is independent of interactions between gC and heparin sulfate on cel surface. All in all , this study demonstrated that the gC of DPV can mediate viral adsorption in an HS-independent manner, which distinguish it from the gC of some other alpha-herpes viruses. Future studies will be required to identify the receptors involved in gC protein binding to cel s. This work provides us a foundation for further studies of examining the roles of gC in the adsorption during duck plague virus infection.
机译：许多哺乳动物疱疹病毒利用细胞表面蛋白聚糖上存在的硫酸乙酰肝素（HS）部分作为细胞进入的受体，并且该过程还需要病毒糖蛋白C（gC）同源物。但是，我们对gC在促进其他α疱疹病毒（如鸭瘟病毒）（DPV）附着中的作用的理解仍是初步的。为了研究gC在DPV感染中的作用，我们使用了缺失gC的突变病毒（DPV-ΔgC-EGFP）。通过实时PCR检查病毒拷贝数，以及对病毒吸附和增殖的时程研究表明，gC参与了病毒与细胞表面的结合。使用原核表达系统和HiTrapTM肝素HP柱色谱法评估病毒糖蛋白（gB-DPV，gC-DPV和gE-DPV）对HS的亲和力。此外，为证实gC在DPV与HS之间的相互作用中起作用，在暴露于DPV-ΔgC-EGFP或野生型中国毒株之前，先用HS类似物肝素处理病毒，并用其抑制剂肝素酶处理宿主细胞。毒鸭瘟病毒（DPV-CHv）。肝素和肝素酶对病毒感染性的影响表明，gC对病毒吸附的功能独立于gC和cel表面上硫酸肝素之间的相互作用。总而言之，这项研究表明DPV的gC可以以HS无关的方式介导病毒吸附，这使其与某些其他α疱疹病毒的gC区别开来。需要进一步的研究来鉴定参与gC蛋白与cel结合的受体。这项工作为我们进一步研究鸭瘟病毒感染期间gC在吸附中的作用提供了基础。

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  • 来源
    《农业科学学报（英文版）》 |2017年第5期|1145-1152|共8页
  • 作者

    JING Yan-chun; YANG Qiao; JING Bo; CHEN Xiao-yue; WU Ying; SUN Kun-feng; WANG Ming-shu; CHENG An-chun; CHEN Shun; JIA Ren-yong; ZHU De-kang; LIU Ma-feng;

  • 作者单位

    Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Avian Diseases Research Center, Col ege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Avian Diseases Research Center, Col ege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Avian Diseases Research Center, Col ege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Avian Diseases Research Center, Col ege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Avian Diseases Research Center, Col ege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Avian Diseases Research Center, Col ege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Avian Diseases Research Center, Col ege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Avian Diseases Research Center, Col ege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Avian Diseases Research Center, Col ege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Avian Diseases Research Center, Col ege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

    Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu 611130, P.R.China;

    Avian Diseases Research Center, Col ege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R.China;

  • 收录信息 中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
  • 原文格式 PDF
  • 正文语种 eng
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