Molecular identification of the cytoplasmic male sterile source from Dongxiang wild rice (Oryza rufipogon Griff.)

YAN Song; ZHU Shan; MAO Ling-hua; HUANG Ren-liang; XIONG Hong-liang; SHEN Lin-jun; SHEN Xian-hua

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Molecular identification of the cytoplasmic male sterile source from Dongxiang wild rice (Oryza rufipogon Griff.)

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The use of cytoplasm male sterility (CMS) is crucial for three-line hybrid seed production.Two types of CMS have been discovered from Dongxiang wild rice,namely the wild abortion type CMS (CMS-WA) and the Dongxiang wild type CMS (CMS-DW).In this study,we show the molecular identification of the two types of CMS in Dongxiang wild rice.WA352,which conferred CMS-WA,was not detected in Dongxiang wild rice,implying Dongxiang wild rice does not carry the CMSWA source.Further analysis of WA352 in DY1A,a CMS-DW line,by PCR amplification and sequencing,revealed two insertion-deletion polymorphisms occurred in CMS-DW compared to WA352 of CMS-WA.It was reported that WA352 was comprised of an unknown origin sequence and partial sequences of three open reading frames (ORFs),orf284,orf224 and orf288.The 42-bp insertion was located between the two segments of orf224 and orf288,which created a new chimeric ORF,orf216.This new ORF was also detected in CMS-HL.Based on the 9-bp deletion in orf284,a specific mitochondrial marker of DW-M1 was developed,which could be used to specifically distinguish the DW-type source.Moreover,semi-quantitative RT-PCR analysis preliminarily suggested that both orf216 and orf284 could be considered as candidates for CMS-DW.These findings present a preliminary understanding of CMS-DW at the molecular level.

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《农业科学学报（英文版）》 |2017年第8期|1669-1675|共7页
作者
YAN Song; ZHU Shan; MAO Ling-hua; HUANG Ren-liang; XIONG Hong-liang; SHEN Lin-jun; SHEN Xian-hua;
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Rice National Engineering Laboratory(Nanchang)/Nanchang Sub-center,National Rice Improvement Center/Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics/Rice Research Institute,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,P.R.China;

Rice National Engineering Laboratory(Nanchang)/Nanchang Sub-center,National Rice Improvement Center/Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics/Rice Research Institute,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,P.R.China;

Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education/College of Agronomy,Jiangxi Agricultural University,Nanchang 330045,P.R.China;

Jiangxi Super-rice Research and Development Center,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,P.R.China;

Rice National Engineering Laboratory(Nanchang)/Nanchang Sub-center,National Rice Improvement Center/Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics/Rice Research Institute,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,P.R.China;

Rice National Engineering Laboratory(Nanchang)/Nanchang Sub-center,National Rice Improvement Center/Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics/Rice Research Institute,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,P.R.China;

Rice National Engineering Laboratory(Nanchang)/Nanchang Sub-center,National Rice Improvement Center/Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics/Rice Research Institute,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,P.R.China;

Rice National Engineering Laboratory(Nanchang)/Nanchang Sub-center,National Rice Improvement Center/Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics/Rice Research Institute,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,P.R.China;

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Molecular identification of the cytoplasmic male sterile source from Dongxiang wild rice (Oryza rufipogon Griff.)

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