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首页> 中文期刊> 《农业科学学报（英文版）》 >Ipomoea batatas HKT1 transporter homolog mediates K+ and Na+uptake in Saccharomyces cerevisiae

Ipomoea batatas HKT1 transporter homolog mediates K+ and Na+uptake in Saccharomyces cerevisiae

         
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摘要

Soil salinity causes the negative effects on the growth and yield of crops. In this study, two sweet potato (Ipomoea batatas L.) cultivars, Xushu 28 (X-28) and Okinawa 100 (O-100), were examined under 50 and 100 mmol L–1 NaCl stress. X-28 cultivar is relatively high salt tolerant than O-100 cultivar. Interestingly, real-time quantitative polymerase chain reaction (RT-qPCR) results indicated that sweet potato high-affinity K+ transporter 1 (IbHKT1) gene expression was highly induced by 50 and 100 mmol L–1 NaCl stress in the stems of X-28 cultivar than in those of O-100 cultivar, but only slightly induced by these stresses in the leaves and fibrous roots in both cultivars. To characterize the function of IbHKT1 transporter, we performed ion-flux analysis in tobacco transient system and yeast complementation. Tobacco transient assay showed that IbHKT1 could uptake sodium (Na+). Yeast complementation assay showed that IbHKT1 could take up K+ in 50 mmol L–1 K+ medium without the presence of NaCl. Moreover, Na+ uptake significantly increased in yeast overexpressing IbHKT1. These results showed that IbHKT1 transporter could have K+-Na+ symport function in yeast. Therefore, the modes of action of IbHKT1 in transgenic yeast could differ from the mode of action of the other HKT1 transporters in class Ⅰ. Potentially, IbHKT1 could be used to improve the salt tolerance nature in sweet potato.

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  • 来源
    《农业科学学报（英文版）》 |2017年第10期|2168-2176|共9页
  • 作者

    PARK Sung-chul; MA Dai-fu; SUN Jian; LI Qiang; YU Yi-cheng; KOU Meng; YAN Hui; TANG Wei; WANG Xin; LIU Ya-ju; ZHANG Yun-gang; KWAK Sang-soo;

  • 作者单位

    Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District/Key Laboratory for Biology and Genetic Breeding of Sweet Potato, Ministry of Agriculture/Sweet Potato Research Institute, Chinese Academy of Agricultural Science, Xuzhou 221131, P.R.China;

    Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District/Key Laboratory for Biology and Genetic Breeding of Sweet Potato, Ministry of Agriculture/Sweet Potato Research Institute, Chinese Academy of Agricultural Science, Xuzhou 221131, P.R.China;

    Institute of Integrative Plant Biology, School of Life Science, Jiangsu Normal University, Xuzhou 221116, P.R.China;

    Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District/Key Laboratory for Biology and Genetic Breeding of Sweet Potato, Ministry of Agriculture/Sweet Potato Research Institute, Chinese Academy of Agricultural Science, Xuzhou 221131, P.R.China;

    Institute of Integrative Plant Biology, School of Life Science, Jiangsu Normal University, Xuzhou 221116, P.R.China;

    Institute of Integrative Plant Biology, School of Life Science, Jiangsu Normal University, Xuzhou 221116, P.R.China;

    Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District/Key Laboratory for Biology and Genetic Breeding of Sweet Potato, Ministry of Agriculture/Sweet Potato Research Institute, Chinese Academy of Agricultural Science, Xuzhou 221131, P.R.China;

    Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District/Key Laboratory for Biology and Genetic Breeding of Sweet Potato, Ministry of Agriculture/Sweet Potato Research Institute, Chinese Academy of Agricultural Science, Xuzhou 221131, P.R.China;

    Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District/Key Laboratory for Biology and Genetic Breeding of Sweet Potato, Ministry of Agriculture/Sweet Potato Research Institute, Chinese Academy of Agricultural Science, Xuzhou 221131, P.R.China;

    Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District/Key Laboratory for Biology and Genetic Breeding of Sweet Potato, Ministry of Agriculture/Sweet Potato Research Institute, Chinese Academy of Agricultural Science, Xuzhou 221131, P.R.China;

    Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District/Key Laboratory for Biology and Genetic Breeding of Sweet Potato, Ministry of Agriculture/Sweet Potato Research Institute, Chinese Academy of Agricultural Science, Xuzhou 221131, P.R.China;

    Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District/Key Laboratory for Biology and Genetic Breeding of Sweet Potato, Ministry of Agriculture/Sweet Potato Research Institute, Chinese Academy of Agricultural Science, Xuzhou 221131, P.R.China;

    Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305806, Republic of Korea;

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