SFRP2 affects prenatal muscle development and is regulated by microRNA-1/206 in pigs

MA Yan-jiao; YANG Ya-lan; SUN Wei; ZHOU Rong; LI Kui; TANG Zhong-lin

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SFRP2 affects prenatal muscle development and is regulated by microRNA-1/206 in pigs

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Secreted frizzled-related protein 2(SFRP2), a member of the SFRPs family, is associated with cell growth and differentiation in myogenesis. Our previous study suggested that SFRP2 was a potential target of micro RNA(mi RNA)-1/206, which was considered as myomiR s. To further explore the biological function and regulation mechanisms of the SFRP2 gene in porcine skeletal muscle development, we first analyzed the sequence structure of the porcine SFRP2 gene. Subsequently, we detected its tissue distribution in adult Tongcheng pigs(a Chinese indigenous breed) and investigated its dynamic expression in developmental skeletal muscle(13 prenatal and 7 postnatal time points) in Tongcheng pigs. An interaction analysis between SFRP2 and myomi Rs was also performed. The results showed that the expression pattern of the SFRP2 varied greatly across diverse tissues. It exhibited abundant expression in prenatal skeletal muscle and peaked at 55 days post coitus(E55), and had a lower expression in postnatal skeletal muscle, indicating that the SFRP2 gene might affect porcine embryonic skeletal muscle development. Co-expression analysis revealed that the expression levels of SFRP2 correlated negatively with mi RNA-1(r=–0.570, P-value=0.009) and mi RNA-206(r=–0.546, P-value=0.013), but positively with SFRP1(r=0.613, P-value=0.004). The bioinformatics analysis and dual luciferase assay verified that the SFRP2 was a putative target of mi RNA-1/206 in pigs. Therefore, this study is helpful for understanding the biological function and molecular regulation of the SFRP2 gene during porcine skeletal muscle development.

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《农业科学学报（英文版）》 |2016年第1期|153-161|共9页
作者
MA Yan-jiao; YANG Ya-lan; SUN Wei; ZHOU Rong; LI Kui; TANG Zhong-lin;
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Jilin Provincial Key Laboratory, College of Animal Science, Jilin University, Changchun 130012, P.R.China;

Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

College of Animal Science and Technology, Northwest A&F University, Yangling 712100, P.R.China;

Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

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SFRP2 affects prenatal muscle development and is regulated by microRNA-1/206 in pigs

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