Identification of the nitrogen-fixing Pseudomonas stutzeri major flagellar gene regulator FleQ and its role in biofilm formation and root colonization

MA Yao; ZHANG Qiu-lei; YANG Zhi-min; LI Yun; YAN Yong-liang; PING Shu-zhen; ZHANG Li-wen

首页> 中文期刊> 《农业科学学报（英文版）》 >Identification of the nitrogen-fixing Pseudomonas stutzeri major flagellar gene regulator FleQ and its role in biofilm formation and root colonization

Identification of the nitrogen-fixing Pseudomonas stutzeri major flagellar gene regulator FleQ and its role in biofilm formation and root colonization

开具论文收录证明 >>

期刊封面封底目录下载 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Flagellar biosynthesis and motility are subject to a four-tiered transcriptional regulatory circuit in Pseudomonas,and the master regulator FleQ appears to be the highest-level regulator in this hierarchical regulatory cascade.Pseudomonas stutzeri A1501 is motile by a polar flagellum;however,the motility and regulatory mechanisms involved in this process are unknown.Here,we searched the A1501 genome for flagella and motility genes and found that approximately 50 genes,which were distributed in three non-contiguous chromosomal regions,contribute to the formation,regulation and function of the flagella.The non-polar mutation of fleQ impaired flagellar biosynthesis,motility and root colonization but enhanced biofilm formation.FleQ positively regulates the expression of flagellar class Ⅱ-Ⅳ genes,suggesting a regulatory cascade that is coordinated similar to that of the well-known P.aeruginosa.Based on our results,we propose that flagellar genes in P.stutzeri A1501 are regulated in a cascade regulated by FleQ and that flagellum-driven motility properties may be necessary for competitive rhizosphere colonization.

著录项

来源
《农业科学学报（英文版）》 |2016年第2期|339-348|共10页
作者
MA Yao; ZHANG Qiu-lei; YANG Zhi-min; LI Yun; YAN Yong-liang; PING Shu-zhen; ZHANG Li-wen;
展开▼
作者单位

College of Biological Sciences,China Agricultural University,Beijing 100094,P.R.China;

Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,P.R.China;

Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,P.R.China;

Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,P.R.China;

The Key Laboratory of Plant Pathology of Hubei Province/College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,P.R.China;

Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,P.R.China;

Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,P.R.China;

Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,P.R.China;

Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,P.R.China;

展开▼
原文格式 PDF
正文语种 eng
中图分类
关键词

Identification of the nitrogen-fixing Pseudomonas stutzeri major flagellar gene regulator FleQ and its role in biofilm formation and root colonization

摘要

著录项

相关主题

期刊订阅