Expression analysis for candidate genes associated with eggshell mechanical property

SUN Cong-jiao; DUAN Zhong-yi; QU Lu-jiang; ZHENG Jiang-xia; YANG Ning; XU Gui-yun

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Expression analysis for candidate genes associated with eggshell mechanical property

机译：与蛋壳力学特性相关的候选基因的表达分析

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Damaged eggshells result in losses of eggs.The genetic mechanism of variable eggshell strength is still unclear.The current study was conducted to verify whether the eggshell calcification related genes,CALB1,SPP1,DMP4,BMP2 and SLIT2,were associated with eggshell mechanical property.For this purpose quantitative PCR(q-PCR) analysis was performed to detect gene expression between two groups of hens laying strong and weak eggs.The hens were selected from 360 White Leghorn layers at 60 wk to ensure that the strong and weak eggs differed significantly in breaking strength but not in eggshell thickness and weight.Using this special strong/weak eggshell model,we found that the expression of CALB1 in the uterus of strong shell group was about 3-fold higher(P<0.05) than that in weak shell group.The DMP4 expression was significantly higher(2-fold,P<0.05) in the uterus of weak shell group than that in strong shell group.However,no difference was observed for genes of SPP1,SLIT2 and BMP2 between these two groups.The current study provides a new insight to investigate the association of candidate genes with eggshell mechanical property.

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来源
《农业科学学报（英文版）》 |2016年第2期|397-402|共6页
作者
SUN Cong-jiao; DUAN Zhong-yi; QU Lu-jiang; ZHENG Jiang-xia; YANG Ning; XU Gui-yun;
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作者单位

National Engineering Laboratory for Animal Breeding/Key Laboratory of Animal Genetics and Breeding,Ministry of Agriculture/College of Animal Science and Technology,China Agricultural University,Beijing 100193,P.R.China;

National Engineering Laboratory for Animal Breeding/Key Laboratory of Animal Genetics and Breeding,Ministry of Agriculture/College of Animal Science and Technology,China Agricultural University,Beijing 100193,P.R.China;

National Engineering Laboratory for Animal Breeding/Key Laboratory of Animal Genetics and Breeding,Ministry of Agriculture/College of Animal Science and Technology,China Agricultural University,Beijing 100193,P.R.China;

National Engineering Laboratory for Animal Breeding/Key Laboratory of Animal Genetics and Breeding,Ministry of Agriculture/College of Animal Science and Technology,China Agricultural University,Beijing 100193,P.R.China;

National Engineering Laboratory for Animal Breeding/Key Laboratory of Animal Genetics and Breeding,Ministry of Agriculture/College of Animal Science and Technology,China Agricultural University,Beijing 100193,P.R.China;

National Engineering Laboratory for Animal Breeding/Key Laboratory of Animal Genetics and Breeding,Ministry of Agriculture/College of Animal Science and Technology,China Agricultural University,Beijing 100193,P.R.China;

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收录信息中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
原文格式 PDF
正文语种 eng
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Expression analysis for candidate genes associated with eggshell mechanical property

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