Isolation and molecular characterization of theFLOWERING LOCUS C gene promoter sequence in radish (Raphanus sativus L.)

XU Yuan-yuan; WANG Jing; NIE Shan-shan; HUANG Dan-qiong; WANG Yan; XU Liang; WANG Rong-hua; LUO Xiao-bo; LIU Li-wang

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Isolation and molecular characterization of theFLOWERING LOCUS C gene promoter sequence in radish (Raphanus sativus L.)

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Both bolting and lfowering times inlfuence taproot and seed production in radish.FLOWERING LOCUS C (FLC) plays a key role in plant lfowering by functioning as a repressor. Two genomic DNA sequences, a 3046-bp from an early- and a 2959-bp from a late-bolting radish line were isolated and named asRsFLC1 andRsFLC2, respectively, for they share approximately 87.03% sequence identity to the FLC cDNA sequences. The genomic DNA sequences, 1466-bp and 1744-bp, lfanking the 5´-regions ofRsFLC1andRsFLC2, respectively, were characterized. Since both of them harbor the basic promoter elements, the TATA box and CAAT box, they were designated asPRsFLC1 andPRsFLC2. The transcription start site (TSS) was identiifed at 424 and 336 bp upstream of the start codon inPRsFLC1 andPRsFLC2,respectively.cis-regulatory elements including CGTCA (MeJA-responsive) and ABRE (abscisic acid-responsive) motifs were found in both promoters, while some cis-regulatory elements including TCA element and GARE-motif were present only inPRsFLC1. These sequence differences lead to the diversity of promoter core elements, which could partialy result in the difference of bolting and lfowering time in radish line NauDY13 (early-bolting) and Naulu127 (late-bolting). Furthermore, to investigate the activity of these promoters, a series of 5´-deletion fragment-GUS fusions were constructed and transformed into tobacco.GUS activity was detected inPRsFLC1-(1 to 4)-GUS-PS1aG-3 andPRsFLC2-(1 to 4)-GUS-PS1aG-3 transgenic tobacco leaf discs, and this activity progressively decreased fromPRsFLC-1-GUS-PS1aG-3 toPRsFLC-5-GUS-PS1aG-3. Deletion analysis indicated that the cis-regulatory elements located at –395 bp to +1 bp may be critical for specifyingRsFLCgene transcription.

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《农业科学学报（英文版）》 |2016年第4期|763-774|共12页
作者
XU Yuan-yuan; WANG Jing; NIE Shan-shan; HUANG Dan-qiong; WANG Yan; XU Liang; WANG Rong-hua; LUO Xiao-bo; LIU Li-wang;
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National Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops East China, Ministry of Agriculture/Colege of Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R.China;

National Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops East China, Ministry of Agriculture/Colege of Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R.China;

National Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops East China, Ministry of Agriculture/Colege of Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R.China;

Department of Plant Sciences, North Dakota State University, Fargo, ND 58108, USA;

National Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops East China, Ministry of Agriculture/Colege of Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R.China;

National Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops East China, Ministry of Agriculture/Colege of Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R.China;

National Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops East China, Ministry of Agriculture/Colege of Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R.China;

National Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops East China, Ministry of Agriculture/Colege of Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R.China;

National Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops East China, Ministry of Agriculture/Colege of Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R.China;

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Isolation and molecular characterization of theFLOWERING LOCUS C gene promoter sequence in radish (Raphanus sativus L.)

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