Transcriptome datasets supply basic gene information for RNAi pest management and gene functional studies inNephotettix cincticeps (Uhler)

CHEN Tai-yu; HOU Ji-xiang; LIN Yong-jun

首页> 中文期刊> 《农业科学学报（英文版）》 >Transcriptome datasets supply basic gene information for RNAi pest management and gene functional studies inNephotettix cincticeps (Uhler)

Transcriptome datasets supply basic gene information for RNAi pest management and gene functional studies inNephotettix cincticeps (Uhler)

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RNA interference (RNAi) technology has the potential to be used in pest management in crop production. Here, the transcriptome ofNephotettix cincticeps(Uhler) was deeply sequenced to investigate the systematic RNAi mechanism and candidate genes for dsRNA feeding. In our datasets, a total of 81225 transcripts were obtained with the length from 150 bp to about 4.2 kb. Almost al the genes related to the RNAi core pathway were proved to be present inN. cincticeps transcriptome. Two transcripts that respectively encode a systemic interference defective (SID) were identiifed in our da-tabase, indicating that the systematic RNAi pathway can function effectively inN. cincticeps. Our datasets not only supply basic gene information for the studies of gene expression and functions in N. cincticeps, such as the control genes for gene expression analysis, but also provide candidate genes for RNAi pest management, such as the genes that encode P450 monooxygenase, V-ATPase and chitin synthase.

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《农业科学学报（英文版）》 |2016年第4期|840-847|共8页
作者
CHEN Tai-yu; HOU Ji-xiang; LIN Yong-jun;
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National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R.China;

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R.China;

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R.China;

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Transcriptome datasets supply basic gene information for RNAi pest management and gene functional studies inNephotettix cincticeps (Uhler)

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