Functional identiifcation of phenazine biosynthesis genes in plant pathogenic bacteriaPseudomonas syringae pv.tomato and Xanthomonas oryzaepv.oryzae

LI Wen; XU You-ping; Jean-Pierre Munyampundu; XU Xin; QI Xian-fei; GU Yuan; CAI Xin-zhong

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Functional identiifcation of phenazine biosynthesis genes in plant pathogenic bacteriaPseudomonas syringae pv.tomato and Xanthomonas oryzaepv.oryzae

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Phenazines are secondary metabolites with broad spectrum antibiotic activity and thus show high potential in biological control of pathogens. In this study, we identiifed phenazine biosynthesis (phz) genes in two genome-completed plant pathogenic bacteriaPseudomonas syringae pv.tomato(Pst) DC3000 andXanthomonas oryzaepv.oryzae(Xoo) PXO99A. Unlike the phz genes in typical phenazine-producing pseudomonads,phz homologs inPst DC3000 andXoo PXO99A consisted of phzC/D/E/F/G andphzC/E1/E2/F/G, respectively, and the both were not organized into an operon. Detection experiments demonstrated that phenazine-1-carboxylic acid (PCA) ofPst DC3000 accumulated to 13.4 μg L–1, while that ofXoo PXO99A was almost undetectable. Moreover,Pst DC3000 was resistant to 1 mg mL–1 PCA, whileXoo PXO99A was sensitive to 50 μg mL–1 PCA. Furthermore, mutation ofphzF blocked the PCA production and signiifcantly reduced the pathogenicity ofPst DC3000 in tomato, while the complementary strains restored these phenotypes. These results revealed thatPst DC3000 produces low level of and is resistant to phenazines and thus is unable to be biologicaly controled by phenazines. Additionaly,phz-mediated PCA production is required for ful pathogenicity ofPst DC3000. To our knowledge, this is the ifrst report of PCA production and its function in pathogenicity of a plant pathogenicP. syringaestrain.

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《农业科学学报（英文版）》 |2016年第4期|812-821|共10页
作者
LI Wen; XU You-ping; Jean-Pierre Munyampundu; XU Xin; QI Xian-fei; GU Yuan; CAI Xin-zhong;
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Institute of Biotechnology, Colege of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China;

Center of Analysis and Measurement, Zhejiang University, Hangzhou 310058, P.R.China;

Institute of Biotechnology, Colege of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China;

Institute of Biotechnology, Colege of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China;

Institute of Biotechnology, Colege of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China;

Institute of Biotechnology, Colege of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China;

Institute of Biotechnology, Colege of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China;

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Functional identiifcation of phenazine biosynthesis genes in plant pathogenic bacteriaPseudomonas syringae pv.tomato and Xanthomonas oryzaepv.oryzae

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