Cloning of theOAT gene and the correlation between its expression and drought tolerance inPhaseolus vulgaris L.

CHEN Ji-bao; CAO Yuan-nan; ZHANG Zhao-yuan; WANG Shu-min; WU Jing; WANG Lan-fen

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Cloning of theOAT gene and the correlation between its expression and drought tolerance inPhaseolus vulgaris L.

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Drought stress is a major abiotic stress of common bean (Phaseolus vulgaris L.) throughout the world. Increasing the proline accumulation contributes to enhance crop drought tolerance. A cDNA for δ-ornithine aminotransferase (δ-OAT), an enzyme involved in the biosynthesis of proline, was isolated fromPhaseolus vulgaris (PvOAT).PvOAT exhibits 87.4 and 39.8% similarity of the deduced amino acid sequences withδ-OAT fromGlycine maxandVigna aconitifolia, respectively. The transcriptional analysis revealed thatPvOAT was strongly induced by drought stress. And the expression ofPvOAT was higher in leaves than that in the root and stem of common bean by drought stress. Similar increase of the proline accumulation was observed in leaves and roots of common bean by drought stress. Furthermore, the proline content, the PvOAT expression and the PvOAT enzyme activity in cultivar F5575 was signiifcantly (P<0.01) higher than that in cultivar F4851 under drought-stress conditions. Interestingly, it had been observed that, in the later stage of drought stress, the proline steadily maintained at the maximum level maybe result from the PvOAT enzyme activity increasing steadily. These results indicated that the expression ofPvOAT and the accumulation of proline induced by drought stress treatment were related to the degree of common bean drought tolerance. So our results support the view that δ-OAT is associated with proline synthesis under drought stress conditions.

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《农业科学学报（英文版）》 |2016年第5期|973-982|共10页
作者
CHEN Ji-bao; CAO Yuan-nan; ZHANG Zhao-yuan; WANG Shu-min; WU Jing; WANG Lan-fen;
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School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, P.R.China;

School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, P.R.China;

School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, P.R.China;

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China;

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China;

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China;

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Cloning of theOAT gene and the correlation between its expression and drought tolerance inPhaseolus vulgaris L.

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