Inducible overexpression of porcine homeobox A10 in the endometrium of transgenic mice

LIN Rui-yi; WU Di; ZHAO Chang-zhi; CHEN Shang-shang; XIAO Qian; LI Xin-yun; ZHAO Shu-hong

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Inducible overexpression of porcine homeobox A10 in the endometrium of transgenic mice

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Homeobox A10 (HOXA10) is a wel-known transcription factor that plays an important role in directing endometrial differ-entiation and establishing the conditions required for implantation. Interestingly, the expression level ofHOXA10 may be associated with litter size. To study the effects of the porcineHOXA10 promoter fragment on the expression ofHOXA10 genein vivo, we generated a transgenic mouse model using pronuclear microinjection, and measured the expression of HOXA10 in the endometrium. There was no difference in the expression level ofHOXA10 between transgenic and wild-type mice in the absence of hormone stimulation. However, folowing treatment with progesterone and estradiol benzoate, the expression level ofHOXA10 was signiifcantly increased in transgenic mice compared with that of wild-type mice. Fur-thermore, the litter size of transgenic females was larger than that of wild-type females (7.02±1.73vs. 6.48±1.85;P=0.14). Moreover, the difference of litter size was greater in the later parities (7.33±1.62vs. 6.37±2.02; P=0.08) compared with the ifrst parity (6.76±1.81vs. 6.61±1.67;P=0.77) between transgenic and wild-type mice. Therefore, our transgenic mouse model provides exciting insights regarding the actions ofHOXA10 and its hormone-inducible promoterin vivo. The present study offers valuable proof of principle to develop transgenic pigs with a hormone-inducible promoter regulatingHOXA10 to alter litter size.

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《农业科学学报（英文版）》 |2016年第6期|1338-1344|共7页
作者
LIN Rui-yi; WU Di; ZHAO Chang-zhi; CHEN Shang-shang; XIAO Qian; LI Xin-yun; ZHAO Shu-hong;
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Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R.China;

Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R.China;

Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R.China;

Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R.China;

Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R.China;

Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R.China;

Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R.China;

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Inducible overexpression of porcine homeobox A10 in the endometrium of transgenic mice

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