In vitro establishment of a highly effective method of castor bean (Ricinus communisL.) regeneration using shoot explants

ZHANG Ji-xing; CHI Yue; CHEN Yong-sheng; WANG Xiao-yu; FENG Zi-zhou; GENG Xue-jun; MU Sha-moli; HUO Hong-yan; TONG Huan; LI Meng-zhu; LI Yi

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In vitro establishment of a highly effective method of castor bean (Ricinus communisL.) regeneration using shoot explants

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An efifcient plant regeneration protocol was established for castor bean (Ricinus communisL.), in which 0.3 mg L–1 thidiazuron (TDZ) induced shoot clusters and increased the number of adventitious shoots from hypocotyl tissue. Our results showed that treatment under dark conditions signiifcantly promoted the average number of shoots per explant to 37.36±4.54 (with a 6-d treatment). Modiifed 1/2 Murashige and Skoog (MS) basal medium supplemented with 440 mg L–1 Ca2+, 0.2 mg L–1 gibberelic acid and 0.1 mg L–1 TDZ signiifcantly increased shoot elongation rates and lowered vitriifcation rates. Further-more, 1/2 MS media supplemented with 0.2 mg L–1 1-naphthaleneacetic acid induced a higher rooting rate compared with other culture conditions.

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《农业科学学报（英文版）》 |2016年第6期|1417-1422|共6页
作者
ZHANG Ji-xing; CHI Yue; CHEN Yong-sheng; WANG Xiao-yu; FENG Zi-zhou; GENG Xue-jun; MU Sha-moli; HUO Hong-yan; TONG Huan; LI Meng-zhu; LI Yi;
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作者单位

School of Life Science, Inner Mongolia University for Nationalities, Tongliao 028000, P.R.China;

School of Life Science, Inner Mongolia University for Nationalities, Tongliao 028000, P.R.China;

School of Life Science, Inner Mongolia University for Nationalities, Tongliao 028000, P.R.China;

Colege of Plant Science, Jilin University, Changchun 130062, P.R.China;

School of Life Science, Inner Mongolia University for Nationalities, Tongliao 028000, P.R.China;

School of Life Science, Inner Mongolia University for Nationalities, Tongliao 028000, P.R.China;

School of Life Science, Inner Mongolia University for Nationalities, Tongliao 028000, P.R.China;

School of Life Science, Inner Mongolia University for Nationalities, Tongliao 028000, P.R.China;

School of Life Science, Nanjing Agricultural University, Nanjing 210095, P.R.China;

School of Life Science, Shandong University, Jinan 25000, P.R.China;

School of Life Science, Shandong University, Jinan 25000, P.R.China;

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In vitro establishment of a highly effective method of castor bean (Ricinus communisL.) regeneration using shoot explants

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