ModiifedBfat-1 gene and its biological veriifcation in mice by hydrodynamic tail vein injection

GAO Xue; DU Xin-hua; ZHANG Lu-pei; CHEN Yan; GAO Hui-jiang; XU Shang-zhong; LI Jun-ya

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ModiifedBfat-1 gene and its biological veriifcation in mice by hydrodynamic tail vein injection

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Omega-3 polyunsaturated fatty acids (ω-3 PUFAs) are essential components required for normal celular function and have been shown to have important therapeutic and nutritional beneifts in humans. But humans or mammals cannot naturaly produce ω-3 PUFAs, due to the lack of the ω-3 fatty acid desaturase gene (fat-1 gene). Previously,fat-1 gene has been cloned fromCaenorhabditis elegans and transferred into mice, pigs and sheep, but not yet into beef cattle. We attempt to transfer it into beef cattle. The object of this paper is to edit thefat-1 gene fromC. elegansto express more efifciently in beef cattle and verify its biological function in mice model. As a result, thefat-1 gene fromC. eleganswas modiifed by synonymous codon usage and named it Bfat-1. We have demonstrated that degree of codon bias ofBfat-1 gene was in-creased in beef cattle. Moreover,Bfat-1gene could be transiently expressed in mouse liver and muscle, the ω-6/ω-3 PUFAs ratio of 18 and 20 carbon was decreased signiifcantly in liver (P<0.05), and the ratio of 20 carbon decreased signiifcantly in muscle 24 and 72 h after injection (P<0.05).This conifrms that theBfat-1gene modiifcation was successful, and the protein encoded was able to catalyze the conversion of ω-6 PUFAs to ω-3 PUFAs.

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《农业科学学报（英文版）》 |2016年第6期|1330-1337|共8页
作者
GAO Xue; DU Xin-hua; ZHANG Lu-pei; CHEN Yan; GAO Hui-jiang; XU Shang-zhong; LI Jun-ya;
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Key Laboratory for Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

Key Laboratory for Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

Key Laboratory for Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

Key Laboratory for Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

Key Laboratory for Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

Key Laboratory for Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

Key Laboratory for Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

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ModiifedBfat-1 gene and its biological veriifcation in mice by hydrodynamic tail vein injection

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