Analysis of cytosine methylation in early generations of resynthesized Brassica napus

RAN Li-ping; FANG Ting-ting; RONG Hao; JIANG Jin-jin; FANG Yu-jie; WANG You-ping

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Analysis of cytosine methylation in early generations of resynthesized Brassica napus

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DNA methylation, an important epigenetic modiifcation, serves as a key function in the polyploidization of numerous crops. In this study, early generations of resynthesizedBrassica napus (F1, S1–S3), ancestral parentsB. rapaandB. oleracea were analyzed to characterize their DNA methylation status during polyploidization, applying DNA methylation-sensitive ampliifca-tion polymorphism (MSAP) and high-performance liquid chromatography methods. InF1, 53.4% fragments were inherited from both A- and C-genomes. Besides, 5.04 and 8.87% fragments inF1 were inherited from A- and C- genome, respectively. 5.85 and 0.8% fragments were newly appeared and disappeared in resynthesizedB. napus, respectively. 13.1% of these gene sites were identiifed with methylation changes inF1, namely, hypermethylation (7.86%) and hypomethylation (5.24%). The lowest methylation status was detected inF1 (38.7%) compared with in S1–S3. In S3, 40.32% genes were methylated according to MSAP analysis. Sequencing of methylated fragments indicated that genes involved in multiple biological processes were modiifed, including transcription factors, protein modiifcation, and transporters. Expression ananlysis of DNA methyltransferase 1 andDNA methyltransferase chromomethylase 3 in different materials was consistent to the DNA methylation status. These results can generaly facilitate dissection of how DNA methylation contributes to genetic stability and improvement ofB. napus during polyploidization.

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《农业科学学报（英文版）》 |2016年第6期|1228-1238|共11页
作者
RAN Li-ping; FANG Ting-ting; RONG Hao; JIANG Jin-jin; FANG Yu-jie; WANG You-ping;
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Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, P.R.China;

Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, P.R.China;

Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, P.R.China;

Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, P.R.China;

Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, P.R.China;

Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, P.R.China;

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Analysis of cytosine methylation in early generations of resynthesized Brassica napus

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