The cellular interactome for glycoprotein 5 of the Chinese highly pathogenic porcine reproductive and respiratory syndrome virus

DU Ji-ge; GE Xin-na; DONG Hong; ZHANG Ning; ZHOU Lei; GUO Xin; YANG Han-chun

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The cellular interactome for glycoprotein 5 of the Chinese highly pathogenic porcine reproductive and respiratory syndrome virus

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摘要

The glycoprotein 5(GP5) of porcine reproductive and respiratory syndrome virus(PRRSV) is a multi-functional protein that plays important roles in virus assembly,entry and viral anti-host responses.In the present study,we investigated the cellular binding partners of GP5 by using lentivirus transduction coupled with immunoprecipitation and mass spectrometry.There were about 40 cellular proteins identified with high Confidence Icons by MS/MS.Ingenuity Pathway Analysis(IPA)indicated that these proteins could be assigned to different functional classes and networks.Furthermore,we validated some of the interactions by co-immunoprecipitation(Co-IP) and confocal microscopy,including those with mitofilin,a mitochondrial inner membrane protein that might be involved in PRRSV or GP5-induced apoptosis,and calnexin,a protein chaperone that might facilitate the folding and maturation of GP5.The interactome data contribute to understand the role and molecular mechanisms of GP5 in PRRSV pathogenesis.

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《农业科学学报（英文版）》 |2016年第8期|1833-1845|共13页
作者
DU Ji-ge; GE Xin-na; DONG Hong; ZHANG Ning; ZHOU Lei; GUO Xin; YANG Han-chun;
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作者单位

Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.China;

Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.China;

Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.China;

Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.China;

Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.China;

Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.China;

Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.China;

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The cellular interactome for glycoprotein 5 of the Chinese highly pathogenic porcine reproductive and respiratory syndrome virus

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