Characterization and function of Tomato yellow leaf curl virus-derived small RNAs generated in tolerant and susceptible tomato varieties

BAI Miao; YANG Guo-shun; CHEN Wen-ting; LIN Run-mao; LING Jian; MAO Zhen-chuan; XIE Bing-yan

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Characterization and function of Tomato yellow leaf curl virus-derived small RNAs generated in tolerant and susceptible tomato varieties

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Virus-tolerant plant,which allows the accumulation of virus and then generates virus-derived small RNAs(vsRNAs),is a valuable material to reveal the antiviral efficiency of vsRNAs.Here,a comparison of vsRNAs in Tomato yellow leaf curl virus tolerant and in susceptible tomato varieties showed the consistent trend of vsRNAs' distribution on virus genome,which is presented as an obvious characteristic.However,the expression level of vsRNA in tolerant variety is less than that in susceptible variety.Slicing targets of vsRNA-mediated viral transcripts were investigated using parallel analysis of RNA ends,and geminivirus DNA methylation was determined by bisulfite sequencing,which uncovered that not all vsRNAs participated in viral mRNA degradation and DNA methylation.Additionally,by comparing with the expression pattern of vsRNAs,viral DNA and mRNA,we proposed the quantity of vsRNAs is corresponding to the expression level of viral mRNA,while the virus-suppression of vsRNAs is not high-efficient.

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《农业科学学报（英文版）》 |2016年第8期|1785-1797|共13页
作者
BAI Miao; YANG Guo-shun; CHEN Wen-ting; LIN Run-mao; LING Jian; MAO Zhen-chuan; XIE Bing-yan;
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College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, P.R.China;

College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, P.R.China;

College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, P.R.China;

Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China;

Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China;

Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China;

Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China;

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Characterization and function of Tomato yellow leaf curl virus-derived small RNAs generated in tolerant and susceptible tomato varieties

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