Overexpression of vacuolar proton pump ATPase (V-H+-ATPase) subunits B, C and H confers tolerance to salt and saline-alkali stresses in transgenic alfalfa (Medicago sativaL.)

WANG Fa-wei; CHEN Xi-feng; WANG Zhen-min; LI Hai-yan; WANG Chao; SUN Yao; WANG Nan; LI Xiao-wei; DONG Yuan-yuan; YAO Na; LIU Xiu-ming; CHEN Huan

首页> 中文期刊> 《农业科学学报（英文版）》 >Overexpression of vacuolar proton pump ATPase (V-H+-ATPase) subunits B, C and H confers tolerance to salt and saline-alkali stresses in transgenic alfalfa (Medicago sativaL.)

Overexpression of vacuolar proton pump ATPase (V-H+-ATPase) subunits B, C and H confers tolerance to salt and saline-alkali stresses in transgenic alfalfa (Medicago sativaL.)

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The vacuolar proton pump ATPase (V-H+-ATPase), which is a multi-subunit membrane protein complex, plays a major role in the activation of ion and nutrient transport and has been suggested to be involved in several physiological processes, such as cel expansion and salt tolerance. In this study, three genes encoding V-H+-ATPase subunits B (ScVHA-B, GenBank: JF826506), C (ScVHA-C, GenBank: JF826507) and H (ScVHA-H, GenBank: JF826508) were isolated from the halophyte Suaeda corniculata. The transcript levels ofScVHA-B,ScVHA-C andScVHA-H were increased by salt, drought and sa-line-alkali treatments. V-H+-ATPase activity was also examined under salt, drought and saline-alkali stresses. The results showed that V-H+-ATPase activity was correlated with salt, drought and saline-alkali stress. Furthermore, V-H+-ATPase subunits B, C and H (ScVHA-B,ScVHA-C andScVHA-H) fromS. corniculata were introduced separately into the alfalfa genome. The transgenic alfalfa was veriifed by Southern and Northern blot analysis. During salt and saline-alkali stresses, transgenic lines carrying the B, C and H subunits had higher germination rates than the wild type (WT). More free proline, higher superoxide dismutase (SOD) activity and lower malondialdehyde (MDA) levels were detected in the transgenic plants under salt and saline-alkali treatments. Moreover, theScVHA-B transgenic lines showed greater tolerance to salt and saline-alkali stresses than the WT. These results suggest that overexpression ofScVHA-B,ScVHA-C andScVHA-H improves tolerance to salt and saline-alkali stresses in transgenic alfalfa.

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《农业科学学报（英文版）》 |2016年第10期|2279-2289|共11页
作者
WANG Fa-wei; CHEN Xi-feng; WANG Zhen-min; LI Hai-yan; WANG Chao; SUN Yao; WANG Nan; LI Xiao-wei; DONG Yuan-yuan; YAO Na; LIU Xiu-ming; CHEN Huan;
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College of Life Sciences, Jilin Agricultural University/Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Changchun 130118, P.R.China;

Jilin Technology Innovation Center for Soybean Region, Jilin Agricultural University, Changchun 130118, P.R.China;

Jilin Technology Innovation Center for Soybean Region, Jilin Agricultural University, Changchun 130118, P.R.China;

College of Life Sciences, Jilin Agricultural University/Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Changchun 130118, P.R.China;

College of Life Sciences, Jilin Agricultural University/Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Changchun 130118, P.R.China;

College of Life Sciences, Jilin Agricultural University/Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Changchun 130118, P.R.China;

College of Life Sciences, Jilin Agricultural University/Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Changchun 130118, P.R.China;

College of Life Sciences, Jilin Agricultural University/Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Changchun 130118, P.R.China;

College of Life Sciences, Jilin Agricultural University/Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Changchun 130118, P.R.China;

College of Life Sciences, Jilin Agricultural University/Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Changchun 130118, P.R.China;

College of Life Sciences, Jilin Agricultural University/Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Changchun 130118, P.R.China;

College of Life Sciences, Jilin Agricultural University/Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Changchun 130118, P.R.China;

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Overexpression of vacuolar proton pump ATPase (V-H+-ATPase) subunits B, C and H confers tolerance to salt and saline-alkali stresses in transgenic alfalfa (Medicago sativaL.)

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