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首页> 中文期刊> 《农业科学学报（英文版）》 >Sperm pretreatment with glutathione improves IVF embryos development through increasing the viability and antioxidative capacity of sex-sorted and unsorted bull semen

Sperm pretreatment with glutathione improves IVF embryos development through increasing the viability and antioxidative capacity of sex-sorted and unsorted bull semen

         
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摘要

The antioxidant of reduced glutathione (GSH) is the most abundant thiol in cels for the maintenance of the intracelular redox balance. The study aimed to assay the effect of sperm treatment with GSH before incubation with oocytes on the development potential of embryos obtained byin vitro fertilization (IVF). Also the mitochondrial membrane potential (ΔΨm), plasma membrane integrity (viability), DNA fragmentation, reactive oxygen species (ROS) content, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities, methane dicarboxylic aldehyde (MDA) level as indices of lipid peroxidation in sex-sorted and unsorted sperm from three buls were investigated using lfow cytometry and enzyme-la-beled instrument individualy. The results showed that 2 mmol L–1 GSH increased signiifcantly the cleavage rate (86.68%vs. 82.78%), 4- to 8-cel rate (82.30%vs. 73.43%) and blastocyst rate (43.15%vs. 35.24%) of IVF embryos compared with untreated group. Furthermore, addition of GSH increased signiifcantly the ΔΨm and viability, decreased the ratio of DNA fragmentation in sex-sorted or unsorted semen (P<0.05), except the sex-sorted semen from bul 019. Similarly, activities of SOD, CAT and GPx were increased signiifcantly. However, the contents of MDA were decreased signiifcantly both in sex-sorted and unsorted semen treated with GSH (P<0.05). These results suggest that sperm pretreatment with GSH during IVF can maintain better the viability and fertility of sperm through reducing apoptosis and increasing the antioxidant capacity, which improves the IVF embryos development.

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  • 来源
    《农业科学学报（英文版）》 |2016年第10期|2326-2335|共10页
  • 作者

    HU Ting-xi; ZHU Hua-bin; SUN Wei-jun; HAO Hai-sheng; ZHAO Xue-ming; DU Wei-hua; WANG Zong-li;

  • 作者单位

    Colege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, P.R.China;

    Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

    Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

    Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

    Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

    Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

    Colege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, P.R.China;

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  • 正文语种 eng
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