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Preparation and utilization of phosphate biofertilizers using agricultural waste

机译：利用农业废弃物制备和利用磷酸盐生物肥料

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摘要

In this study,Aspergilus niger 1107 was isolated and identiifed as an efifcient phosphate-solubilizing fungus (PSF). This strain generated 689 mg soluble P L–1 NBRIP medium after 10 d of culture. To produce an affordable biofertilizer using A. niger 1107, the potential of widely available carrier materials for growth and maintenance of this strain were evaluated. The effects of sterilization procedures (autoclaving and gamma-ray irradiation) on the suitability of these carriers to maintain growth of the fungus were also investigated. The carrier materials were peat, corn cobs with 20% (w/w) perlite (CCP), wheat husks with 20% (w/w) perlite (WHP), and composted cattle manure with 20% (w/w) perlite (CCMP). In the ifrst 5-6 mon of storage, the carriers sterilized by gamma-ray irradiation maintained higher inoculum loads than those in carriers sterilized by autoclaving. However, this effect was not detectable after 7 mon of storage. For the P-biofertilizer on WHP, more than 2.0×107 viable spores ofA. niger g–1 inoculantsurvived after 7 mon of storage. When this biofertilizer was applied to Chinese cabbage in a pot experiment, there were 5.6×106spores ofA. niger g–1 soil before plant harvesting. In the pot experiment, Chinese cabbage plants grown in soil treated with peat- and WHP-based P-biofertilizers showed signiifcantly greater growth (P<0.05) than that of plants grown in soil treated with free-cel biofertilizer or the CCMP-based biofertilizer. Also, the peat- and WHP-based P-biofertilizers increased the available P content in soil.
机译：在这项研究中，黑曲霉1107被分离并鉴定为有效的磷酸盐增溶真菌（PSF）。培养10 d后，该菌株产生了689 mg可溶性PL-1 NBRIP培养基。为了使用黑曲霉1107生产负担得起的生物肥料，评估了广泛可用的载体材料对该菌株的生长和维持的潜力。还研究了灭菌程序（高压灭菌和伽马射线辐照）对这些载体保持真菌生长的适应性。载体材料为泥炭，含20％（w / w）珍珠岩（CCP）的玉米芯，含20％（w / w）珍珠岩（WHP）的小麦壳和含20％（w / w）珍珠岩的堆肥牛粪（ CCMP）。在储存的前5-6个月中，通过伽马射线辐照灭菌的载体比通过高压灭菌灭菌的载体保持更高的接种量。但是，在储存7个月后无法检测到这种效果。对于WHP上的P型生物肥料，A的活孢子超过2.0×107。储存7个月后，尼日尔g–1接种物存活。在盆栽试验中，将这种生物肥料应用于大白菜时，有5.6×106个A孢子。收获植物前，尼日尔g–1土壤。在盆栽试验中，在以泥炭和WHP为基础的P-生物肥料处理过的土壤中生长的大白菜植物，其生长速度显着高于在以自由cel生物肥料或CCMP为基础的生物肥料处理过的土壤中生长的植物（P <0.05）。 。而且，基于泥炭和WHP的P生物肥提高了土壤中的有效P含量。

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  • 来源
    《农业科学学报（英文版）》 |2015年第1期|158-168|共11页
  • 作者

    WANG Hong-yuan; LIU Shen; ZHAI Li-mei; ZHANG Ji-zong; REN Tian-zhi; FAN Bing-quan; LIU Hong-bin;

  • 作者单位

    Key Laboratory of Nonpoint Source Pollution Control, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China;

    Key Laboratory of Nonpoint Source Pollution Control, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China;

    Key Laboratory of Nonpoint Source Pollution Control, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China;

    Key Laboratory of Nonpoint Source Pollution Control, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China;

    Key Laboratory of Nonpoint Source Pollution Control, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China;

    Key Laboratory of Nonpoint Source Pollution Control, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China;

    Key Laboratory of Nonpoint Source Pollution Control, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China;

  • 收录信息 中国科学引文数据库(CSCD);
  • 原文格式 PDF
  • 正文语种 eng
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