Effects of root restriction on nitrogen and gene expression levels in nitrogen metabolism in Jumeigui grapevines (Vitis viniferaL.×Vitis labruscaL.)

YU Xiu-ming; LI Jie-fa; ZHU Li-na; WANG Bo; WANG Lei; BAI Yang; ZHANG Cai-xi; XU Wen-ping; WANG Shi-ping

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Effects of root restriction on nitrogen and gene expression levels in nitrogen metabolism in Jumeigui grapevines (Vitis viniferaL.×Vitis labruscaL.)

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To decipher the relationship between the inhibited shoot growth and expression pattern of key enzymes in nitrogen metabolism under root restriction, the effects of root restriction on diurnal variation of expression of nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS1-1,GS1-2,GS2) and glutamate synthase (Fd-GOGAT,NADH-GOGAT) genes and nitrogen levels were evaluated in two-year-old Jumeigui grapevines (Vitis viniferaL.×Vitis labruscaL.) when signiifcant differences in shoot growth were observed between treatments at expansion stage (22 days after anthesis). Grapevines were planted in root-restricting pits as root restriction and in an unrestricted ifeld as the control. Results showed that root restriction signiifcantly reduced shoot growth, but promoted the growth of white roots and ifbrous brown roots and improved the fruit quality. (NO3–+NO2–)-N concentration in al plant parts, NH4+-N concentration in white roots and total N concentration in leaves and brown roots were signiifcantly reduced under root restriction. Gene expression analysis revealed that mRNA levels of genes related to the GS1/NADH-GOGAT pathway were lower in root-restricted than in control petioles, whereas genes involved in the GS2/Fd-GOGAT pathway were up-regulated under root restriction. Root restriction also resulted in down-regulation of genes involved in nitrogen metabolism in leaves, especialy at 10:00, while transcript levels of al these genes were enhanced in root-restricted white and brown roots at most time points. This organ-dependent response contributed to the alteration in NO3– reduction and NH4+ assimilation under root restriction, leading to less NO3– transported from roots and then assimilated in root-restricted leaves. Therefore, this study implied that shoot growth inhibition in grapevines under root restriction is closely associated with down-regulation of gene expression in nitrogen metabolism in leaves.

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《农业科学学报（英文版）》 |2015年第1期|67-80|共14页
作者
YU Xiu-ming; LI Jie-fa; ZHU Li-na; WANG Bo; WANG Lei; BAI Yang; ZHANG Cai-xi; XU Wen-ping; WANG Shi-ping;
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Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P.R.China;

Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P.R.China;

Shanghai Academy of Agricultural Sciences, Shanghai 201106, P.R.China;

Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P.R.China;

Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P.R.China;

Guangxi Academy of Agricultural Sciences, Nanning 530007, P.R.China;

Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P.R.China;

Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P.R.China;

Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P.R.China;

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Effects of root restriction on nitrogen and gene expression levels in nitrogen metabolism in Jumeigui grapevines (Vitis viniferaL.×Vitis labruscaL.)

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