• 主题
高级搜索  >
历史搜索 清空历史
首页> 中文期刊> 《农业科学学报（英文版）》 >Identiifcation of the miniature pig inbred line by skin allograft

Identiifcation of the miniature pig inbred line by skin allograft

         
页面导航
  • 摘要
  • 著录项
  • 相关主题

摘要

Skin grafting has been used as one of the most reliable tests to determine the genetic stability of laboratory animal such as mice and rats inbred line, but no identiifcation of swine inbred lines by skin grafting has been reported. At present, Wuzhishan miniature pig (WZSP) inbred line has acquired the F24 individuals in China. In order to verify whether WZSP inbred line had been cultivated successful y, al ogeneic skin grafts and related research were performed on F20 individuals of WZSP inbreeding population, compared with a control group of autologous transplantation. We observed the transplant recipients’ wounds, detected peripheral blood-related indicators interleukin-2, 4 and 10, CD4+and CD8+lymphocytes, and conducted hematoxylin-eosin (HE) and Masson’s staining of skin to judge whether the immune rejection reactions occurred within 28 days after transplantation. Chr. 7 genomic heterozygosity of 48 WZSP individuals from F20 to F22 was analyzed by high-density single nucleotide polymorphism (SNP) chips (60 000 SNPs). The result showed that there were no signiifcant differences in graft skin, the plasma interleukin-2, 4, 10, CD4+and CD8+, HE and Masson’s staining results between the al ograft and autograft groups, and no immune rejection occurred on the al ograft group. We found that 11 genes in Chr. 7 of major histocompatibility complex (MHC) I and MHC II were homozygous which conifrmed that immune antibody of the al ograft and autograft groups were highly identical and also provided a theoretical basis to no immune rejection occurred on the al ograft in the inbred WZSP. The result proved that the WZSP inbred line had been cultivated successful y for the ifrst time in the world. The test methods also provide a scientiifc basis for the identiifcation of swine and mammal inbred lines.

著录项

  • 来源
    《农业科学学报（英文版）》 |2015年第7期|1376-1382|共7页
  • 作者

    MU Yu-lian; WEI Jing-liang; TANG Fang; YANG Shu-lin; WU Zhi-gu; XIA Ying; SUN Tong-zhu; LIU Lan; FENG Shu-tang; WU Tian-wen; LI Kui; LI Jun-you; HE Wei; GAO Qian; ZHOU Wen-fang;

  • 作者单位

    Key Laboratory of Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

    Key Laboratory of Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

    Key Laboratory of Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

    Key Laboratory of Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

    Key Laboratory of Wound Repair, the General Hospital of the People’s Liberation Army, Beijing 100048, P.R.China;

    Key Laboratory of Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

    Key Laboratory of Wound Repair, the General Hospital of the People’s Liberation Army, Beijing 100048, P.R.China;

    Key Laboratory of Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

    Key Laboratory of Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

    Key Laboratory of Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

    Key Laboratory of Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

    Key Laboratory of Wound Repair, the General Hospital of the People’s Liberation Army, Beijing 100048, P.R.China;

    Key Laboratory of Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

    Key Laboratory of Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

    Key Laboratory of Wound Repair, the General Hospital of the People’s Liberation Army, Beijing 100048, P.R.China;

  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号