iTRAQ quantitative analysis of plasma proteome changes of cow from pregnancy to lactation

MA Lu; BU Deng-pan; YANG Yong-xing; YAN Su-mei; WANG Jia-qi

首页> 外文期刊>农业科学学报（英文版） >iTRAQ quantitative analysis of plasma proteome changes of cow from pregnancy to lactation

【24h】

iTRAQ quantitative analysis of plasma proteome changes of cow from pregnancy to lactation

机译：从怀孕到哺乳期奶牛血浆蛋白质组变化的iTRAQ定量分析

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Dairy cows undergo tremendous changes in physiological, metabolism and the immune function from pregnancy to lac-tation that are associated with cows being susceptible to metabolic and infectious diseases. The objective of this study is to investigate the changes of plasma proteome on 21 d before expected calving and 1 d after calving from dairy cows using an integrated proteomic approach consisting of minor abundance protein enrichment by ProteoMiner beads, protein labeling by isobaric tags for relative and absolute quantiifcation, and protein identiifcation by liquid chromatography coupled with tandem mass spectrometry. Nineteen proteins were changed around the time of calving. These proteins were asso-ciated with response to stress, including acute-phase response and defense response, based on the proteins annotation. In particular, three up-regulated proteins after calving including factor V,α2-antiplasmin and prothrombin were assigned into the complement and coagulation pathway. These results may provide new information in elucidating host response to lactation and parturition stress, and inlfammatory-like conditions at the protein level. Differential proteins may serve as potential markers to regulate the lactation and parturition stress in periparturient dairy cows.

机译：从怀孕到哺乳期，奶牛的生理，新陈代谢和免疫功能都发生了巨大变化，这与易患新陈代谢和传染病的奶牛有关。这项研究的目的是使用一种综合蛋白质组学方法，研究奶牛产犊前21天和产犊后1天血浆蛋白质组的变化，该蛋白质组学包括通过ProteoMiner磁珠富集少量丰度蛋白质，通过等压标记对相对和相对蛋白质进行标记液相色谱和串联质谱联用对蛋白质进行绝对定量和蛋白质鉴定。产犊时改变了19种蛋白质。这些蛋白质根据蛋白质注释与对应激的反应相关，包括急性期反应和防御反应。特别地，将产犊后三个上调的蛋白（包括因子V，α2-抗纤溶酶和凝血酶原）分配到补体和凝血途径中。这些结果可能为阐明宿主对蛋白质水平上的泌乳和分娩应激以及类似发炎状态的反应提供新的信息。差异蛋白可以作为调节围产期奶牛泌乳和分娩应激的潜在标志物。

著录项

来源
《农业科学学报（英文版）》 |2015年第7期|1407-1413|共7页
作者
MA Lu; BU Deng-pan; YANG Yong-xing; YAN Su-mei; WANG Jia-qi;
展开▼
作者单位

Inner Mongolia Agricultural University, Huhhot 010018, P.R.China;

State Key Laboratory of Animal Nutrition/CAAS-ICRAF Joint Laboratory on Agroforestry and Sustainable Animal Husbandry/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

State Key Laboratory of Animal Nutrition/CAAS-ICRAF Joint Laboratory on Agroforestry and Sustainable Animal Husbandry/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

Inner Mongolia Agricultural University, Huhhot 010018, P.R.China;

State Key Laboratory of Animal Nutrition/CAAS-ICRAF Joint Laboratory on Agroforestry and Sustainable Animal Husbandry/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

展开▼
收录信息中国科学引文数据库(CSCD);
原文格式 PDF
正文语种 eng
中图分类
关键词

iTRAQ quantitative analysis of plasma proteome changes of cow from pregnancy to lactation

摘要

著录项

相关主题

期刊订阅