Effect of the C.-1 388 A>G polymorphism in chicken heat shock transcription factor 3 gene on heat tolerance

ZHANG Wen-wu; KONG Li-na; ZHANG De-xiang; JI Cong-liang; ZHANG Xi-quan; LUO Qing-bin

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Effect of the C.-1 388 A>G polymorphism in chicken heat shock transcription factor 3 gene on heat tolerance

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Heat stress is one of the main factors that inlfuence poultry production. Heat shock proteins (HSPs) are known to affect heat tolerance. The formation of HSPs is regulated by heat shock transcription factor 3 (HSF3) in chicken. A DNA pool was established for identifying single nucleotide polymorphisms (SNPs) of the chicken HSF3, and 13 SNPs were detected. The bioinformatic analysis showed that 8 SNPs had the capacity to alter the transcription activity of HSF3. The dual luciferase report gene assay showed that there was a signiifcant difference (P<0.01) in the Firelfy luciferase/Renil a luciferase ratio (F/R) of C.–1 703 A>G (S1) and C.–1 388 A>G (S4) sites at the 5´-untranslated region (UTR) of chicken HSF3. The elec-trophoretic mobility shift assay showed that the S4 site was a transcription binding factor. The analysis of the association of the S1 and S4 sites with heat tolerance index revealed that the S4 site was signiifcantly correlated with the CD3+T cel , corticosterone, and T3 levels in Lingshan chickens and with the heterophil/lymphocyte value in White Recessive Rock. These results showed that the S4 site at the 5´ UTR of chicken HSF3 might have an impact on heat tolerance in summer and could be used as a potential marker for the selection of chicken with heat tolerance in the future.

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《农业科学学报（英文版）》 |2015年第9期|1808-1815|共8页
作者
ZHANG Wen-wu; KONG Li-na; ZHANG De-xiang; JI Cong-liang; ZHANG Xi-quan; LUO Qing-bin;
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Col ege of Animal Science, South China Agricultural University, Guangzhou 510642, P.R.China;

Col ege of Animal Science, South China Agricultural University, Guangzhou 510642, P.R.China;

Col ege of Animal Science, South China Agricultural University, Guangzhou 510642, P.R.China;

Guangdong Wens Food Group Limited Company, Xinxing 527439, P.R.China;

Col ege of Animal Science, South China Agricultural University, Guangzhou 510642, P.R.China;

Col ege of Animal Science, South China Agricultural University, Guangzhou 510642, P.R.China;

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Effect of the C.-1 388 A>G polymorphism in chicken heat shock transcription factor 3 gene on heat tolerance

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