Efifciency of Different Methods for dsRNA Delivery in Cotton Bollworm (Helicoverpa armigera)

YANG Jing; HAN Zhao-jun

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Efifciency of Different Methods for dsRNA Delivery in Cotton Bollworm (Helicoverpa armigera)

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RNAi trigged by dsRNA not only facilitates the development of molecular biology, but also initiates a new way for pest control by silence of fatal genes. However, one of the key limitations in pest control is lack of the convenient and efifcient method for dsRNA delivery. In this study, different dsRNA delivery methods at their own optimum conditions were evaluated comparatively for their efifciency with Helicoverpa armigera as test animal. It was found that the popular one-time injection of larvae with dsRNA could reduce the pupation rate by 43.0%and enhance larva mortality by 11.7%. One-time ingestion of dsRNA did not result in any signiifcant effect on phenotype. Continuous ingestion of in vitro synthesized dsRNA by refreshing the bait diet every day caused 40.4% decrease in successful pupation and 10.0% increase in larval mortality, which was similar as one-time injection. The most efifcient method was found to be the continuous ingestion of the bacteria containing dsRNA expressed, which reduced the rate of pupation by 68.7%and enhanced the larval mortality by 34.1%. Further analysis found that dsRNA was degraded faster in midgut juice than in hemolymph. However, the cell of bacteria could protect dsRNA and delay the degradation in the midgut juice of H. armigera. These results throw light on the application of dsRNA in pest management with proper ways.

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《农业科学学报（英文版）》 |2014年第1期|115-123|共9页
作者
YANG Jing; HAN Zhao-jun;
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Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education/College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, P.R.China;

Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education/College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, P.R.China;

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Efifciency of Different Methods for dsRNA Delivery in Cotton Bollworm (Helicoverpa armigera)

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