The Minimal Active Fragment of the Cry1Ai Toxin is Located Between 36I and 605I

ZHOU Zi-shan; LIN Hui-yan; LI Ying; SHU Chang-long; SONG Fu-ping; ZHANG Jie

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The Minimal Active Fragment of the Cry1Ai Toxin is Located Between 36I and 605I

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The novel cry1Ai gene that cloned from Bacillus thuringiensis strain SC6H8 encoded a protein exhibiting strong toxicity against Plutella xylostella and Chilo suppressalis in our previous study. Using the available information for the active fragments of other Cry toxins, eight truncated fragments were constructed to identify the minimal active fragment of Cry1Ai. All truncated fragments were expressed in Escherichia coli strain BL21 (DE3), and the insecticidal activity against 2nd-instar P. xylostella larvae was assessed using full-length Cry1Ai as a positive control. The results indicate that the minimal active fragment of the Cry1Ai toxin against P. xylostella is located between amino acid residues 36I and 605I, which is smaller than the regions previously reported for Cry1A. The ifrst two amino acids (34T and 35P) on helixα-1 and whole helixα-2 of domain I and sheetβ-32 of domain III are necessary for Cry1Ai toxin to keep its toxicity against P. xylostella.

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《农业科学学报（英文版）》 |2014年第5期|1036-1042|共7页
作者
ZHOU Zi-shan; LIN Hui-yan; LI Ying; SHU Chang-long; SONG Fu-ping; ZHANG Jie;
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State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;

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The Minimal Active Fragment of the Cry1Ai Toxin is Located Between 36I and 605I

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