Functional Analysis of the Phosphoenolpyruvate Carboxylase on the Lipid Accumulation of Peanut (Arachis hypogaea L.) Seeds

PAN Li-juan; YU Shan-lin; YANG Qing-li; CHI Xiao-yuan; CHEN Ming-na; YANG Zhen; CHEN Na; WANG Tong; WANG Mian; HE Ya-nan

首页> 外文期刊>农业科学学报（英文版） >Functional Analysis of the Phosphoenolpyruvate Carboxylase on the Lipid Accumulation of Peanut (Arachis hypogaea L.) Seeds

【24h】

Functional Analysis of the Phosphoenolpyruvate Carboxylase on the Lipid Accumulation of Peanut (Arachis hypogaea L.) Seeds

机译：磷酸烯醇丙酮酸羧化酶对花生种子（花生）脂质沉积的功能分析

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Phosphoenolpyruvate carboxylase (PEPC;EC 4.1.1.31) catalyses phosphoenolpyruvate (PEP) to yield oxaloacetate, which is involved in protein biosynthesis. Pyruvate kinase (PK;EC 2.7.1.40) catalyzes PEP to yield pyruvate, which is involved in fatty acid synthesis. In this study, five PEPC genes (AhPEPC1, AhPEPC2, AhPEPC3, AhPEPC4, and AhPEPC5) from peanut have been cloned. Using a quantitative real-time RT-PCR approach, the expression pattern of each gene was monitored during the seed development of four peanut varieties (E11, Hebeigaoyou, Naihan 1, and Huayu 26). It was found that these five genes shared similar expression behaviors over the developmental stages of E11 with high expression levels at 30 and 40 d after pegging (DAP);whereas these five genes showed irregular expression patterns during the seed development of Hebeigaoyou. In Naihan 1 and Huayu 26, the expression levels of the five genes remained relatively high in the first stage. The PEPC activity was monitored during the seed development of four peanut varieties and seed oil content was also characterized during whole period of seed development. The PEPC activity followed the oil accumulation pattern during the early stages of development but they showed a significantly negative correlation thereafter. These results suggested that PEPC may play an important role in lipid accumulation during the seed development of four peanut varieties tested.

机译：磷酸烯醇丙酮酸羧化酶（PEPC; EC 4.1.1.31）催化磷酸烯醇丙酮酸（PEP）生成草酰乙酸，其参与蛋白质的生物合成。丙酮酸激酶（PK; EC 2.7.1.40）催化PEP产生丙酮酸，丙酮酸参与脂肪酸的合成。在这项研究中，从花生中克隆了五个PEPC基因（AhPEPC1，AhPEPC2，AhPEPC3，AhPEPC4和AhPEPC5）。使用定量实时RT-PCR方法，在四个花生品种（E11，河北高邮，乃汉1和华玉26）的种子发育过程中监测每个基因的表达模式。结果发现，这五个基因在E11的发育阶段都表现出相似的表达行为，且在钉住（DAP）后30和40 d具有高表达水平;而这五个基因在河北高邮种子发育过程中表现出不规则的表达模式。在奈汉1号和华豫26号中，五个基因的表达水平在第一阶段仍保持较高水平。在四个花生品种的种子发育过程中监测了PEPC的活性，并在整个种子发育过程中对种子油含量进行了表征。在开发的早期阶段，PEPC的活性遵循油的聚集模式，但此后它们显示出显着的负相关性。这些结果表明，PEPC可能在测试的四个花生品种的种子发育过程中在脂质积累中发挥重要作用。

著录项

来源
《农业科学学报（英文版）》 |2013年第1期|36-44|共9页
作者
PAN Li-juan; YU Shan-lin; YANG Qing-li; CHI Xiao-yuan; CHEN Ming-na; YANG Zhen; CHEN Na; WANG Tong; WANG Mian; HE Ya-nan;
展开▼
作者单位

Shandong Peanut Research Institute, Qingdao 266100, P.R.China;

Shandong Peanut Research Institute, Qingdao 266100, P.R.China;

Shandong Peanut Research Institute, Qingdao 266100, P.R.China;

Shandong Peanut Research Institute, Qingdao 266100, P.R.China;

Shandong Peanut Research Institute, Qingdao 266100, P.R.China;

Shandong Peanut Research Institute, Qingdao 266100, P.R.China;

Shandong Peanut Research Institute, Qingdao 266100, P.R.China;

Shandong Peanut Research Institute, Qingdao 266100, P.R.China;

Shandong Peanut Research Institute, Qingdao 266100, P.R.China;

Shandong Peanut Research Institute, Qingdao 266100, P.R.China;

展开▼
收录信息中国科学引文数据库(CSCD);
原文格式 PDF
正文语种 eng
中图分类
关键词

Functional Analysis of the Phosphoenolpyruvate Carboxylase on the Lipid Accumulation of Peanut (Arachis hypogaea L.) Seeds

摘要

著录项

相关主题

期刊订阅