Expression of Sex-Related Genes in Chicken Embryos During Male-to-Female Sex Reversal Exposure to Diethylstilbestrol

FANG Li-xiu; XIN Rui; CHE Yi; XU Shi-qing

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Expression of Sex-Related Genes in Chicken Embryos During Male-to-Female Sex Reversal Exposure to Diethylstilbestrol

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Sex emerges out of a delicate dance between a variety of promale, anti-male, and possibly profemale genes. To investigate the role that sex-related genes play in sex determination and gonadal differentiation of fowl, we constructed a male-to-female sex-reversal model of chick induced by diethylstilbestrol (DES) at onset of incubation (E0). The results of semi-quantitative PCR showed that the expression of Sf1, the orphan nuclear receptor steroidogenic factor-1 gene, was put forward from E7d to E5d and up-regulated during E5-7d;the Dmrt1, the double sex and the Mab-3 related to transcription factor 1 gene, was down-regulated during E3-7d. Meanwhile, anti-Müllerian hormone gene (Amh) expressed at a similar level in the genetic females and sex-reversal females before E7d, while no expression products of the three female-specific genes Wpkci, Fet1 and Foxl2 were detected in male-to-female embryos. These findings suggest that the expression of some certain sex-related genes, induced by the exogenous estrogen during period of sex determination and gonadal differentiation, results in the male-to-female sex reversal. Moreover, high activity of Sf1 gene during E5-7d might be related to the profemale process, while low activity of Dmrt1 gene during E3-5d might be anti-male. The expression activity of Amh gene might only contribute to the promale process after E7d, however, it is possibly not an anti-female gene in chick embryos.

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《农业科学学报（英文版）》 |2013年第1期|127-135|共9页
作者
FANG Li-xiu; XIN Rui; CHE Yi; XU Shi-qing;
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Department of Applied Biology, Medical College, Soochow University, Suzhou 215123, P.R.China;

Department of Applied Biology, Medical College, Soochow University, Suzhou 215123, P.R.China;

Department of Applied Biology, Medical College, Soochow University, Suzhou 215123, P.R.China;

Department of Applied Biology, Medical College, Soochow University, Suzhou 215123, P.R.China;

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Expression of Sex-Related Genes in Chicken Embryos During Male-to-Female Sex Reversal Exposure to Diethylstilbestrol

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