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Characterization of MicroRNA* Species in Peking Duck Skin

         
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摘要

A substantial fraction of miRNA*species are conserved in animals and can repress activities of target genes. This study aims to investigate the miRNA*species in duck skin by using Solexa sequencing. We obtained a total of 96 miRNA*species in two skin small RNA libraries and identified 56 miRNA/miRNA*(miR/miR*) pairs. Nucleotide bias of miRNA*indicated that the priority was C>A>U>G for the first nucleotide and U>C>A>G for the last nucleotide. Comparison analyses showed that 3′-U accounted for a higher proportion in the 56 miR/miR*pairs. Among the top 20 expressed miRNA*species, 17 were shared by two libraries and most of the miRNA*species were highly conservative, especially in the“seed region”. miR-199a*were expressed highly in our samples, which was also previously shown abundant in mouse hair follicle. Furthermore, four miRNA*species were predicted to target their genes in signal pathways of feather follicle development and feather morphogenesis despite very low levels.

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  • 来源
    《农业科学学报（英文版）》 |2013年第9期|1614-1619|共6页
  • 作者

    ZHANG Li; ZHANG Xi-quan; XIE Xiu-juan; JIA Shan-gang; XIAO Mei; LIN Shu-dai; AN Li-long; LUO Wen; JIA Xin-zheng; NIE Qing-hua;

  • 作者单位

    Agricultural College, Guangdong 0cean University, Zhanjiang 524088, P.R.China;

    Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, P.R.China;

    Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, P.R.China;

    Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100029, P.R.China;

    Agricultural College, Guangdong 0cean University, Zhanjiang 524088, P.R.China;

    Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, P.R.China;

    Agricultural College, Guangdong 0cean University, Zhanjiang 524088, P.R.China;

    Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, P.R.China;

    Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, P.R.China;

    Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, P.R.China;

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