The Finding and Phylogenetic Evolution Analysis of Bovine Piroplasms in the Rasŏn Area of North Korea

JIA Li-jun; ZHANG Shou-fa; CAO Shi-nuo; QIAN Nian-chao; YU Long-zheng; XUAN Xue-nan

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The Finding and Phylogenetic Evolution Analysis of Bovine Piroplasms in the Rasŏn Area of North Korea

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The objective of this study was to investigate the epidemiology of bovine Piroplasms infections in the Rasŏn area of North Korea. The survey was carried out by light microscopic examination of Giemsa-stained blood smears, PCR, and phylogenetic evolution analysis of 128 blood samples collected from the Rasŏn area. The results showed that the infection rates of the small and large parasites were about 2.5 and 1.5% on average, respectively, in all Theileria sergenti and Babesia ovata-positive blood smears by microscopic examination of blood smears. The detection rate of T. sergenti Giemsa-stained smears was 43.75%, while that with PCR was 67.97%. The detection rate of B. ovata Giemsa-stained smears was 49.21%, while that with PCR was 71.88%. The sequence and phylogenetic analysis of DNA showed 98.84%homology between the 18S rRNA gene sequences of T. sergenti isolates from North Korean and that of Yanbian state from China, indicating the closest genetic relationship between both of them. Moreover, 100%homology was shown between the 18S rRNA gene sequence of B. ovata isolates from North Korea and the published sequence AY081192 of GenBank, indicating the closest genetic relationship between both of them. This survey confirmed that Rasŏn is the endemic area of T. sergenti and B. ovata in North Korea.

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《农业科学学报（英文版）》 |2013年第10期|1847-1854|共8页
作者
JIA Li-jun; ZHANG Shou-fa; CAO Shi-nuo; QIAN Nian-chao; YU Long-zheng; XUAN Xue-nan;
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Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Yanbian University, Yanji 133002, P.R.China;

Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Yanbian University, Yanji 133002, P.R.China;

National Research Center for Protozoan Diseases, 0bihiro University of Agriculture and Veterinary Medicine, Inada-cho, 0bihiro, Hokkaido 080-8555, Japan;

Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Yanbian University, Yanji 133002, P.R.China;

Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Yanbian University, Yanji 133002, P.R.China;

National Research Center for Protozoan Diseases, 0bihiro University of Agriculture and Veterinary Medicine, Inada-cho, 0bihiro, Hokkaido 080-8555, Japan;

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The Finding and Phylogenetic Evolution Analysis of Bovine Piroplasms in the Rasŏn Area of North Korea

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