cDNA Cloning of Heat Shock Protein Genes and Their Expression in an Indigenous Cryptic Species of the Whitefly Bemisia tabaci Complex from China

YU Hao; WAN Fang-hao; GUO Jian-ying

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cDNA Cloning of Heat Shock Protein Genes and Their Expression in an Indigenous Cryptic Species of the Whitefly Bemisia tabaci Complex from China

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Thermal adaptation plays a fundamental role in shaping the distribution and abundance of insects,and heat shock proteins(Hsps)play important roles in the temperature adaptation of various organisms.To better understand the temperature tolerance of the indigenous ZHJ2-biotype of whitefly Bemisia tabaci species complex,we obtained complete cDNA sequences for hsp90,hsp70,and hsp20 and analyzed their expression profiles under different high temperature treatments by real-time quantitative polymerase chain reaction.The high temperature tolerance of B.tabaci ZHJ2-biotype was determined by survival rate after exposure to different high temperatures for 1 h.The results showed that after 41°C heat-shock treatment for 1 h,the survival rates of ZHJ2 adults declined significantly and the estimated temperature required to cause 50% mortality(LT50)is 42.85°C for 1 h.Temperatures for onset(Ton)or maximal(Tmax)induction of hsps expression in B.tabaci ZHJ2-biotype were 35 and 39°C(or 41°C).Compared with previous studies,indigenous ZHJ2-biotype exhibits lower heat temperature stress tolerance and Ton(or Tmax)than the invasive B-biotype.

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《农业科学学报（英文版）》 |2012年第2期|293-302|共10页
作者
YU Hao; WAN Fang-hao; GUO Jian-ying;
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State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China;

Department of Entomology, Henan Institute of Science and Technology, Xinxiang 453003, P.R. China;

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China;

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China;

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cDNA Cloning of Heat Shock Protein Genes and Their Expression in an Indigenous Cryptic Species of the Whitefly Bemisia tabaci Complex from China

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