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首页> 中文期刊> 《农业科学学报（英文版）》 >Controlled Freezing and Open-Pulled Straw (OPS) Vitrification of In vitro Produced Bovine Blastocysts Following Analysis of ATP Content and Reactive Oxygen Species (ROS) Level

Controlled Freezing and Open-Pulled Straw (OPS) Vitrification of In vitro Produced Bovine Blastocysts Following Analysis of ATP Content and Reactive Oxygen Species (ROS) Level

         
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摘要

To our knowledge,no single study has systemically compared cryopreservation efficiencies of bovine blastocysts derived from in vitro fertilization (IVF),intracytoplasmic sperm injection (ICSI) and somatic cell nuclear transfer (SCNT) by controlled freezing and vitrification.This experiment,therefore,was designed to compare the cryopreservation of these blastocysts with controlled freezing and OPS vitrification.Adenosine-5'-triphosphate (ATP) content and reactive oxygen species (ROS) level in blastocysts were also analyzed.Firstly,for each type of blastocyst (IVF,ICSI or SCNT),significant differences were observed between the survival rates of the controlled freezing ((81.56±2.33),(68.18±4.72) or (47.89±5.83)%) and OPS vitrification groups ((92.24±4.54),(82.40±3.76) or (78.71±5.91)%;P<0.05).Secondly,for each type of blastocyst (IVF,ICSI or SCNT),ATP content was significantly decreased after controlled freezing or vitrification,and the ATP content in the controlled freezing group (0.43±0.06),(0.35±0.05) or (0.21±0.02) pmol) was significantly lower than that found in the OPS vitrification group (0.62±0.04),(0.46±0.03) or (0.30±0.01) pmol;P<0.05).Thirdly,ROS level in fresh IVF ((47.33±3.56) c.p.s (counted photons per second),ICSI ((36.51±2.58) c.p.s) or SCNT blastocysts ((26.44±1.49) c.p.s) was significantly lower than that found in the OPS vitrification group ((72.14±4.31),(58.89±3.89) or (40.11±5.73) c.p.s;P<0.05),but higher than that of the controlled freezing group (34.41±3.32),(23.13±1.26) or (15.46±2.45) c.p.s;P<0.05).The present study indicated that vitrification is more efficient in the cryopreservation of bovine blastocysts derived from IVF,ICSI or SCNT than controlled freezing.Furthermore,both vitrification and controlled freezing significantly altered the ATP content and ROS level in those blastocysts.

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  • 来源
    《农业科学学报（英文版）》 |2012年第3期|446-455|共10页
  • 作者

    ZHAO Xue-ming; DU Wei-hua; WANG Dong; HAO Hai-sheng; QIN Tong; LIU Yan; ZHU Hua-bin;

  • 作者单位

    Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science IAS, Chinese Academy of Agricultural Sciences CAAS, Beijing 100193, P.R.China;

    Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science IAS, Chinese Academy of Agricultural Sciences CAAS, Beijing 100193, P.R.China;

    Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science IAS, Chinese Academy of Agricultural Sciences CAAS, Beijing 100193, P.R.China;

    Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science IAS, Chinese Academy of Agricultural Sciences CAAS, Beijing 100193, P.R.China;

    Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science IAS, Chinese Academy of Agricultural Sciences CAAS, Beijing 100193, P.R.China;

    Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science IAS, Chinese Academy of Agricultural Sciences CAAS, Beijing 100193, P.R.China;

    Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science IAS, Chinese Academy of Agricultural Sciences CAAS, Beijing 100193, P.R.China;

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