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首页> 中文期刊> 《农业科学学报（英文版）》 >Expression of Interleukin-6 and Interleukin-6 Receptor in Ovine Oocytes During In vitro Maturation

Expression of Interleukin-6 and Interleukin-6 Receptor in Ovine Oocytes During In vitro Maturation

         
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摘要

To study the effects of interleukin-6 (IL-6) and its receptor (IL-6R) during in vitro maturation of ovine oocytes, the mRNA and protein expression levels of IL-6 and IL-6R, along with their localization, were examined during ovine oocytes maturation in vitro through real-time PCR, Western blotting, and immunohistochemistry. Specific patterns of expression of IL-6 and IL-6R were observed at both mRNA and protein levels at each stage of ovine oocytes maturation. IL-6 and IL-6R were distributed primarily on the surface of the cell membrane, with little expression in the cytoplasm or nucleus. IL-6 and IL-6R were expressed significantly at higher levels in the maturation around 4 h, and then decreased dramatically. However the level slightly elevated at 20-24 h. The role of IL-6 and IL-6R on oocytes maturation was studied through in vitro addition of recombinant human IL-6 in different concentrations. The addition of 10 ng mL-1 IL-6 significantly increased the rates of oocytes maturation (P<0.05), but did not affect the rates of development of the subsequence IVF ovine embryos. In summary, IL-6 is likely to play an important role in the early ovine oocytes maturation. The expression patterns of the IL-6 and IL-6R on the ovine oocytes maturation open up the possibility of regulatory role of the cytokine in ovine oocytes maturation.

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  • 来源
    《农业科学学报（英文版）》 |2012年第8期|1333-1339|共7页
  • 作者

    ZHAO Xi-an; CANG Ming; GAO Xiao-yu; YANG Mei-ling; YUAN Jian-long; ZHU Bing; WANG Zhi-gang; LIU Dong-jun;

  • 作者单位

    Key Laboratory of Ministry of Education of China for Mammal Reproduction Biology and Biotechnology, Inner Mongolia University,Hohhot 010021, P.R.China;

    Key Laboratory of Ministry of Education of China for Mammal Reproduction Biology and Biotechnology, Inner Mongolia University,Hohhot 010021, P.R.China;

    Key Laboratory of Ministry of Education of China for Mammal Reproduction Biology and Biotechnology, Inner Mongolia University,Hohhot 010021, P.R.China;

    Key Laboratory of Ministry of Education of China for Mammal Reproduction Biology and Biotechnology, Inner Mongolia University,Hohhot 010021, P.R.China;

    Key Laboratory of Ministry of Education of China for Mammal Reproduction Biology and Biotechnology, Inner Mongolia University,Hohhot 010021, P.R.China;

    Key Laboratory of Ministry of Education of China for Mammal Reproduction Biology and Biotechnology, Inner Mongolia University,Hohhot 010021, P.R.China;

    Key Laboratory of Ministry of Education of China for Mammal Reproduction Biology and Biotechnology, Inner Mongolia University,Hohhot 010021, P.R.China;

    Key Laboratory of Ministry of Education of China for Mammal Reproduction Biology and Biotechnology, Inner Mongolia University,Hohhot 010021, P.R.China;

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