A Simplified Seed Transformation Method for Obtaining Transgenic Brassica napus Plants

SONG Li; ZHAO De-gang; WU Yong-jun; TIAN Xiao-e

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A Simplified Seed Transformation Method for Obtaining Transgenic Brassica napus Plants

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We report here a seed transformation of sonication-assisted, no-tissue culture to rapidly produce transgenic Brassica napus plants. This method comprises the steps of treating seeds by ultrasonic wave, inoculating Agrobacterium tumefaciens with a recombinant ChIFN-α gene and germinating directly of treatment seed on wet filter papers. The obtained transformants were verified by GUS histochemical assay and nested PCR amplification. It suggests that seed transformation has a potential use in genetic transformation of rape.

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《农业科学学报：英文版》 |2009年第6期|658-663|共6页
作者
SONG Li; ZHAO De-gang; WU Yong-jun; TIAN Xiao-e;
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作者单位

Guizhou Key Laboratory of Agricultural Bioengineering;

Guizhou University;

Guiyang 550025;

P.R.China;

Key Laboratory of Green Pesticide and Agricultural Biological Engineering;

Ministry of Education/Guizhou University;

Guiyang 550025;

P.R. China;

College of Life Sciences;

Guizhou University;

Guiyang 550025;

P.R.China;

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原文格式 PDF
正文语种 chi
中图分类油菜籽（芸薹）;植物细胞遗传学;
关键词
转基因油菜; 甘蓝型油菜; 种子; 变换方法; 根癌农杆菌介导; 植物; 遗传转化; PCR扩增;

A Simplified Seed Transformation Method for Obtaining Transgenic Brassica napus Plants

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