Transgenic Rape with hrf2 Gene Encoding HarpinXooc Resistant to Sclerotinia sclerotinorium

MA Ling-li; HUO Rong; GAO Xue-wen; HE Dan; SHAO Min; WANG Qi

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Transgenic Rape with hrf2 Gene Encoding HarpinXooc Resistant to Sclerotinia sclerotinorium

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The objective of this study was to increase the resistance of rape using the method of transformation. The gene hrf2 derived from Xathomonas oryzae pv. oryzicola encoding harpinXooc protein was constructed into transgenic vector pCAMBIA1301. The cotyledonal petiole segments from rapeseed variety Yangyou 4 were infected by Agrobacterium tumefaciens strain LBA4404/pCAMBIA1301-hrf2. Hygromycin-resistant green shoots were obtained. Successful integration of the foreign gene into the genome of the rapeseed variety Yangyou 4 was confirmed by PCR, RT-PCR, and β-glucuronidase analyses. Disease bioassays of transgenic plants revealed an improved resistance of transgenic plants to Rape sclerotiniose. In brief, the hrf2 gene can be transferred into rape using the method of Agrobacterium-medmted transformation, which increased the resistance to Sclerotinia sclerotinorium in the transgenic plant.

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《农业科学学报（英文版）》 |2008年第4期|455-461|共7页
作者
MA Ling-li; HUO Rong; GAO Xue-wen; HE Dan; SHAO Min; WANG Qi;
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作者单位

Key Laboratory of Monitoring and Management of Plant Diseases and Pests, Ministry of Agriculture/Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, P.R.China;

Key Laboratory of Monitoring and Management of Plant Diseases and Pests, Ministry of Agriculture/Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, P.R.China;

Key Laboratory of Monitoring and Management of Plant Diseases and Pests, Ministry of Agriculture/Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, P.R.China;

Key Laboratory of Monitoring and Management of Plant Diseases and Pests, Ministry of Agriculture/Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, P.R.China;

Key Laboratory of Monitoring and Management of Plant Diseases and Pests, Ministry of Agriculture/Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, P.R.China;

Key Laboratory of Monitoring and Management of Plant Diseases and Pests, Ministry of Agriculture/Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, P.R.China;

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中图分类农业基础科学;
关键词
hrf2 gene, Agrobacterium tumefaciens-mediated transformation, transgenic oilseed rape, resistance;

机译：hrf2基因;根癌农杆菌介导的转化;转基因油菜;抗性;

Transgenic Rape with hrf2 Gene Encoding HarpinXooc Resistant to Sclerotinia sclerotinorium

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