In this study,the insecticidal active substance of the fruiting body of Paecilomyces cicadae (Miquel) Samson that can kill the larvae of Plutella xylostella (Lepidoptera: Plutellidae) was isolated and purified by combined separation with biology experiments for the first time. By means of bioactive guided isolation of 40% ethanol-H2O,dichloromethane,ethyl acetate,n-butyl ethanol,macroporous adsorption resin column,high performance liquid chromatography,and sephadex A-20 column,the insecticidal active compound was purified,and a white powder compound was obtained. The compound was active against the third instar larvae of P. xylostella in 24,48,and 72 h at the concentration of 10.0 mg mL-1 with a corrective mortality of (95.1 ± 1.8),(97.7 ± 1.6),and (99.1 ± 1.7)%,respectively. The compound was strongly sensitive to the larvae. It was stable at acid environment,and was not sensitive to pepsin,and other chracteristics were studied.
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