Agrobacterium-mediated Transformation of Rice (Oryza sativa L.) with Atrazine Chlorohydrolase Gene (atzA)

WANGSong-wen; SHILi-li; SUNZong-xiu; CAIBao-li; FUYa-ping; WANGYang; SIHua-min; LIUXia; ZHANGXin

首页> 中文期刊> 《农业科学学报：英文版》 >Agrobacterium-mediated Transformation of Rice (Oryza sativa L.) with Atrazine Chlorohydrolase Gene (atzA)

Agrobacterium-mediated Transformation of Rice (Oryza sativa L.) with Atrazine Chlorohydrolase Gene (atzA)

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摘要

Atrazine chlorohydrolase gene (atzA) was cloned from Arthrobacter sp. AD1. A plant expression plasmid was constructed under the control of CaMV35s promoter and was used in rice transformation. The target gene was successfully introduced into mature embryos of a japonica rice cultivar Jindao 107 by Agrobacterium- mediated transformation and hundreds of transgenic plants were obtained. The exogenous atzA gene in the transgenic plants that expressed atrazine resistance was confirmed by Southern blot hybridization. The resistance experiments by spraying transgenic rice plants with 0.133% atrazine shown that most of the transgenic rice plants exhibited the resistance to herbicide atrazine. The segregation of exogenous atzA gene in T1 progeny corresponded to the Mendelian ratio.

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《农业科学学报：英文版》 |2005年第4期|241-246|共6页
作者
WANGSong-wen; SHILi-li; SUNZong-xiu; CAIBao-li; FUYa-ping; WANGYang; SIHua-min; LIUXia; ZHANGXin;
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作者单位

TianjinAgricultralCollege;

Tianjin300384;

P.R.China;

TianjinAgricultralCollege;

Tianjin300384;

P.R.China／／KeyLaboratoryforRiceBiology;

MinistryofAgriculture／ChinaNationalRiceResearchInstitute;

Hangzhou310006;

P.R.China;

KeyLaboratoryforRiceBiology;

MinistryofAgriculture~ChinaNationalRiceResearchInstitute;

Hangzhou310006;

P.R.China;

InstituteofMolecularBiology;

NankaiUniversity;

Tianjin300071;

P.R.China;

KeyLaboratoryforMolecularPhytophathology;

MinistryofAgriculture／ChinaAgriculturalUniversity;

Beijing100094;

P.R.China;

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原文格式 PDF
正文语种 chi
中图分类稻;杂草防治理论与方法;
关键词
转基因水稻; 莠去津; 氯化羟基水解酶基因; 除草剂; 土壤杆菌介导;

机译：转基因水稻;Arthrobacter sp.。 AD1;阿特拉津氯水解酶基因;

Agrobacterium-mediated Transformation of Rice (Oryza sativa L.) with Atrazine Chlorohydrolase Gene (atzA)

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