Analysis of SSR in Citrus Sequences from EMBL Database

MENG Hai-jun; CAO Qing-qin; HU Zhi-yong; LIU Gao-ping; CHENG Yun-jiang; DENG Xiu-xin

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Analysis of SSR in Citrus Sequences from EMBL Database

机译：从EMBL数据库分析柑橘序列中的SSR

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Abundance of simple sequence repeat (SSR) in Citrus sequences from EMBL database was investigated by using computer program MISA (MIcroSAtellite), which aimed to provide useful information for the development of SSR markers.Among 32 896 sequences of Citrus, 4987 SSRs were found in 4167 sequences and the average distance between SSRs was approximately 3.5 kb. Mononucleotide repeats (50.6%) were the most abundant repeats. And di-, tri-, tetra-, penta- and hexa-nucleotide repeats were 22.8, 25.2, 1, 0.08, and 0.36%, respectively. The most abundant motif was A/T followed in descending order by AG/CT, AC/GT, AT/TA. AAT/ATT, AAG/CTT, AGC/CGT, ACG/CTG and C/G. They comprised about90% of all microsatellites. Ten primer pairs were designed, and three of them produced clear visible bands among Citrus and its related genera.

机译：利用计算机程序MISA（MIcroSAtellite）研究了EMBL数据库中柑橘序列中的简单序列重复序列（SSR）的丰富度，旨在为开发SSR标记提供有用的信息。在32个896个柑橘序列中，发现了4987个SSR。 4167个序列，SSR之间的平均距离约为3.5 kb。单核苷酸重复序列（50.6％）是最丰富的重复序列。并且二核苷酸，三核苷酸，四核苷酸，五核苷酸和六核苷酸重复分别为22.8、25.2、1、0.08和0.36％。最丰富的图案是A / T，其次是AG / CT，AC / GT，AT / TA。 AAT / ATT，AAG / CTT，AGC / CGT，ACG / CTG和C / G。它们约占所有微卫星的90％。设计了十对引物，其中三对在柑橘及其相关属之间产生了清晰可见的条带。

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《农业科学学报（英文版）》 |2005年第7期|501-506|共6页
作者
MENG Hai-jun; CAO Qing-qin; HU Zhi-yong; LIU Gao-ping; CHENG Yun-jiang; DENG Xiu-xin;
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National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R. China;

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R. China;

Department of Biotechnology, Beijing Agricultural College, Beijing 102206, P.R. China;

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R. China;

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R. China;

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R. China;

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R. China;

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正文语种 chi
中图分类农学（农艺学）;
关键词
Citrus; Simple sequence repeats; Abundance; Markers;

机译：柑橘;简单的序列重复;丰度;标记;

Analysis of SSR in Citrus Sequences from EMBL Database

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