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Serial Nuclear Transfer of Goat-Rabbit Interspecies Reconstructed Embryos

机译:山羊-兔种间重组胚的系列核转移。

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摘要

The experiments of serial nuclear transfer were conducted between Boer goat and rabbit. The enucleated oocytes of rabbit were used as recipients while the blastomeres of goat morula was used as nuclear donor. The reconstructed embryos developing to morula were used as donor for serial cloning. As a result, two generations of reconstructed embryos were obtained, including 58 first generation reconstructed embryos and 14 second generation reconstructed embryos. The fusion rates were 79.5 and 70%, respectively. and there was no significant difference between them (P>0.05). The cleavage rates were 75.9 and 28.6% respectively with significant difference (P<0.01). No blastocyst was obtained from the second generation reconstructed embryos while 13.8% of first generation reconstructed embryos developed to blastocyst.
机译:在布尔山羊和兔子之间进行了连续核转移的实验。兔去核卵母细胞用作受体,山羊桑ula的卵裂球用作核供体。将发育为桑ula的重构胚用作供体进行系列克隆。结果,获得了两代重建胚,包括58个第一代重建胚和14个第二代重建胚。融合率分别为79.5和70%。两者之间无显着性差异(P> 0.05)。卵裂率分别为75.9和28.6%,差异有统计学意义(P <0.01)。从第二代重建胚中未获得胚泡,而第一代重建胚中有13.8%发育为胚泡。

著录项

  • 来源
    《农业科学学报(英文版)》 |2005年第8期|629-633|共5页
  • 作者单位

    College of of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, P.R. China;

    Medical Reproduction Centre of the First Affiliated Hospital, Anhui Medical University, Hefei 230022, P.R. China;

    College of of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, P.R. China;

    College of of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, P.R. China;

    College of Life Science, University of Science and Technology of China, Hefei 230022, P.R. China;

    College of of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, P.R. China;

    College of of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, P.R. China;

    College of of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, P.R. China;

    College of of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, P.R. China;

    College of of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, P.R. China;

    College of of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, P.R. China;

    College of of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, P.R. China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 农业基础科学;
  • 关键词

    Goat; Interspecies cloning; Serial nuclear transfer;

    机译:山羊;种间克隆;串行核转移;
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