Inhibition of HSP70 Gene Expression by Modified Antisense and Its Effects on Embryonic Sensitivity to Heat Shock

TIANWen-ru; DULi-yin; HEJian-bin; LIShou-jun

首页> 中文期刊> 《农业科学学报：英文版》 >Inhibition of HSP70 Gene Expression by Modified Antisense and Its Effects on Embryonic Sensitivity to Heat Shock

Inhibition of HSP70 Gene Expression by Modified Antisense and Its Effects on Embryonic Sensitivity to Heat Shock

开具论文收录证明 >>

期刊封面封底目录下载 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Experiments were performed to evaluate the efficiency of inhibition of HSP70 gene expressionby antisense oligonucleotides complementary to the mRNA of HSP70 and to test the effects ofinhibition of HSP70 gene expression on subsequent embryonic sensitivity to heat shock. Theresults showed that transfection of pre-implantation embryos at 4-cell stage with 5 Mantisense oligo had no effect on in vitro blastocyst development. However, transfection with10 to 40 M antisense oligo had reduced in vitro blastocyst development to 15, 10% and 0; Forthe embryos which exposed to 40 M As arrested at the 16-cell stage, there was no blastocystformation within the heat shock groups. In contrast, transfection had no effect on embryonicsensitivity to heat shock, above 25% of embryos developed to blastocyst stage in controlgroups.

著录项

来源
《农业科学学报：英文版》 |2004年第2期|149-155|共7页
作者
TIANWen-ru; DULi-yin; HEJian-bin; LIShou-jun;
展开▼
作者单位

FacultyofAnimalScienceandVeterinaryMedicine;

LaiyangAgriculturalCollege;

Laiyang265200;

P.R.China;

AnimalScienceandTechnologyCollege;

InnerMongoliaUniversityforNationalities;

Tongliao028042;

P.R.China;

ShenyangAgriculturalUniversity;

Shenyang110161;

P.R.China;

FacultyofVeterinaryMedicine;

HuananAgriculturalUniversity;

Guangzhou510642;

P.R.China;

展开▼
原文格式 PDF
正文语种 chi
中图分类 S823.2;
关键词
HSP70基因; 基因抑制; 表达; 牛; 胚胎; 热休克; mRNA;

机译：牛胚胎;修饰反义;对HSP70基因的抑制;

Inhibition of HSP70 Gene Expression by Modified Antisense and Its Effects on Embryonic Sensitivity to Heat Shock

摘要

著录项

相关主题

期刊订阅