A Study on the Inhibitory Potency of Protease Inhibitorsin Porcine Colostrum

ZHOUQi; HERui-guo; LIXiang; LIAOSheng-rong; ZHOUWu; DUJin-ping; KONGNi-jia

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A Study on the Inhibitory Potency of Protease Inhibitorsin Porcine Colostrum

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Porcine colostrum and milk were separated into the acid-soluble fraction (SF) and casein frac-tion (CF) by centrifuge. Trypsin and chymotrypsin inhibitory capacity in porcine colostrum, milk and theircomponents were determined by incubating bovine trypsin or chymotrypsin in a medium. The inhibition of in-sulin-like growth factor Ⅰ (IGF-Ⅰ) and epidermal growth factor (EGF) degradation in pig small intestinal con-tents by porcine colostrum was measured by incubating iodinated IGF-Ⅰ or EGF. Degradation of labeled IGF-Ⅰor EGF was determined by monitoring the generation of radioactivity soluble in 30% trichloroacetic acid(TCA). The results showed that porcine colostrum had high levels of trypsin and chymotrypsin inhibitory ac-tivity and increased the stability of IGF-Ⅰ and EGF in pig intestinal contents. The SF was higher in inhibitorypotency than CF. The present study revealed that the protease inhibitors in porcine colostrum, milk-derivedand colostrum-specific, existed mainly in SF.

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《农业科学学报：英文版》 |2003年第7期|809-814|共6页
作者
ZHOUQi; HERui-guo; LIXiang; LIAOSheng-rong; ZHOUWu; DUJin-ping; KONGNi-jia;
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作者单位

CollegeofAnimalScience;

HuazhongAgriculturalUniversity;

Wuhan430070;

P.R.China;

HubeiAcademyofAgriculturalSciences;

Wuhan430064;

P.R.China;

MedicineCollege;

HuazhongScienceandTechnologyUniversity;

Wuhan430080;

19.R.China;

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原文格式 PDF
正文语种 chi
中图分类 S828.1;
关键词
猪; 初乳; 牛奶; 蛋白酶抑制剂; 生长因子; 离心机; 胰岛素; 糜蛋白酶;

机译：猪初乳;蛋白酶抑制剂;抑制能力;生长因子;

A Study on the Inhibitory Potency of Protease Inhibitorsin Porcine Colostrum

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