Identification of Intergeneric Hybrid Plants Between Oryza sativa and O. minuta via GISH and RAPD

YU Shun-wu; CHEN Bao-tang; TAO Ai-lin; ZHANG Duan-pin

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Identification of Intergeneric Hybrid Plants Between Oryza sativa and O. minuta via GISH and RAPD

机译：利用GISH和RAPD鉴定水稻和小稻之间的属间杂交植物

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To transfer desirable resistance traits from O. minuta to O. sativa, intergeneric hybrid plants between O. sativa (AA, 2n=2X=24) and O. minuta (BBCC, 2n=4X=48) were produced by embryo rescue after sexual cross. Morphological observation and chromosome counts indicated their hybrid status (ABC, 2n =3X=36). Genomic in situ hybridization (GISH) was further applied to confirm the parentage of the chromosomes of F1 hybrids. Chromosomes of O. minuta and O. sativa were distinguishable in the hybrids in different fluorescence colors. GISH indicated that A and BC chromosomes were not randomly assembled in a cell.RAPD profiles unequivocally revealed their hybrids with double parent patterns. The results of blast tests showed that the hybrids had obtained disease resistance from O. minuta, and had a level of susceptibility between the parents.

机译：从O. Minuta转移到O. Sativa的理想抗性性状，在胚胎（aa，2n = 2x = 24）和o. minuta（bbcc，2n = 4x = 48）之间产生的邻羟杂交植物是通过胚胎救援在性行为后产生的叉。形态学观察和染色体计数表明它们的杂化状态（ABC，2N = 3x = 36）。进一步应用原位杂交（GISH）的基因组，以确认F1杂交种的染色体的父母。 O. Minuta和O. Sativa的染色体在不同荧光颜色的杂种中可区分。 GISH表明A和BC染色体未在细胞中随机组装.RAPD型材明确地揭示其具有双母图案的杂种。爆破试验结果表明，杂交种已获得O. Minuta的抗病性，并且在父母之间具有敏感性。

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《农业科学学报（英文版）》 |2003年第12期|1297-1301|共5页
作者
YU Shun-wu; CHEN Bao-tang; TAO Ai-lin; ZHANG Duan-pin;
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作者单位

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P. R. China;

Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200030, P. R. China;

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P. R. China;

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P. R. China;

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P. R. China;

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中图分类植物学;
关键词
Genomic in situ hybridization; Hybrid; Oryza minuta; Oryza sativa; Blast;

机译：基因组原位杂交;杂交;Oryza minuta;Oryza sativa;爆炸;

Identification of Intergeneric Hybrid Plants Between Oryza sativa and O. minuta via GISH and RAPD

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