Cloning and Characterization of a PGIP-Encoding Gene from Solanum torvum

ZHANG Fan; WANG Zhong; HUANG Quan-sheng; HUANG Le-ping; YANG Qing

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Cloning and Characterization of a PGIP-Encoding Gene from Solanum torvum

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Verticillium wilt is a severe disease in eggplant caused by Verticillium dahliae.Polygalacturonase-inhibiting proteins (PGIPs) have been shown to be involved in preventing the invasion of fungus including V.dahliae.Cloning genes encoding PGIPs is quite valuable for plant resistance breeding to Verticillium wilt.In this study,a cDNA encoding the polygalacturonase-inhibiting protein was isolated from Solanum torvum by RT-PCR and RACE,designated StPGIP (accession no.FJ943498).The cDNA sequence of StPGIP was 1 097 bp long and contained an open reading frame of 990 bp.The predicted amino acid sequence of the gene consisted of 329 amino acids and had conserved LRRs.The StPGIP protein had a high identity with PGIPs from other species.Analysis of StPGIP expression at the mRNA level by RT-PCR showed that the gene was expressed in all organs and could be induced to increase expression by V.erticillium dahliae infection.

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《农业科学学报：英文版》 |2010年第6期|921-927|共7页
作者
ZHANG Fan; WANG Zhong; HUANG Quan-sheng; HUANG Le-ping; YANG Qing;
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作者单位

State Key Laboratory of Crop Genetics and Germplasm Enhancement;

College of Life Sciences;

Nanjing Agricultural University;

Nanjing 210095;

P.R. China;

Institute of Nuclear and Biotechnology;

Xinjiang Academy of Agricultural Sciences;

Urmuqi 830091;

P.R.China;

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原文格式 PDF
正文语种 chi
中图分类转化及克隆;马铃薯（土豆）;
关键词
编码基因; 马铃薯; 蛋白质; PCR检测; 克隆; 抗黄萎病; RACE技术; cDNA序列;

Cloning and Characterization of a PGIP-Encoding Gene from Solanum torvum

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