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首页> 中文期刊> 《农业科学学报（英文版）》 >Mycoplasma leachii causes bovine mastitis: Evidence from clinical symptoms, histopathology and immunohistochemistry

Mycoplasma leachii causes bovine mastitis: Evidence from clinical symptoms, histopathology and immunohistochemistry

         
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摘要

Twelve quarters of six lactating cows were inoculated with Mycoplasma leachii strain GN407 through intramammary ductal infusion; another 12 quarters were inoculated with heat-inactivated M. leachii culture medium as negative controls. Multidisciplinary procedures, including clinical assessment, etiology assessment, pathology and immunohistochemistry (IHC), were used to elucidate the pathogenicity of M. leachii in bovine mastitis. From post-inoculation days (PIDs) 3 to 9, 12 inoculated quarters developed mild to severe clinical mastitis and mammary tissue histopathological changes, including inflammatory cell infiltration and architectural destruction of mammary gland ducts. The M. leachii antigen was also detected by IHC in the mammary gland epithelial cells of the inoculated quarters as a weak signal on PID 6 and as a strong signal on PID 9. The control quarters also developed mild mastitis and histopathological changes on PID 9, and M. leachii was also detected by IHC. Throughout the experimental period, the quarters of the negative control cow were clinically and pathologically normal, and the M. leachii antigen was not detected. In conclusion, direct histological and immunohistochemical evidence confirmed that M. leachii causes clinical bovine mastitis through histopathological lesions induced by invasion of the pathogen into mammary gland cells and through inflammatory cell infiltration.

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  • 来源
    《农业科学学报（英文版）》 |2019年第1期|160-168|共9页
  • 作者

    CHANG Ji-tao; YU Li; YU De-bin; LIANG Jian-bin; CHEN Jia; WANG Jian-fa; WANG Fang; JIANG Zhi-gang; HE Xi-jun; WU Rui;

  • 作者单位

    Division of Livestock Infectious Diseases/State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.China;

    Division of Livestock Infectious Diseases/State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.China;

    College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University (Daqing High-tech Industrial Development Zone), Daqing 163319, P.R.China;

    College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University (Daqing High-tech Industrial Development Zone), Daqing 163319, P.R.China;

    College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University (Daqing High-tech Industrial Development Zone), Daqing 163319, P.R.China;

    College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University (Daqing High-tech Industrial Development Zone), Daqing 163319, P.R.China;

    Division of Livestock Infectious Diseases/State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.China;

    Division of Livestock Infectious Diseases/State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.China;

    Pathology Laboratory/State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.China;

    College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University (Daqing High-tech Industrial Development Zone), Daqing 163319, P.R.China;

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