Integrating transcriptomic and proteomic analyses of photoperiod-sensitive in near isogenic maize line under long-day conditions

SONG Xiao-heng; TIAN Lei; WANG Shun-xi; ZHOU Jin-Iong; ZHANG Jun; CHEN Zan; WU Liu-ji; KU Li-xia; CHEN Yan-hui

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Integrating transcriptomic and proteomic analyses of photoperiod-sensitive in near isogenic maize line under long-day conditions

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As a short-day plant species, maize requires an optimal photoperiod for inducing reproductive growth. However, there is a lack of information regarding photoperiod-induced changes in maize mRNA and protein levels. In this study, a photoperiod-insensitive maize inbred line and its near isogenic photoperiod-sensitive line were used. By integrating RNA-based transcriptomic and iTRAQ LC-MS/MS-based proteomic approaches, we generated a comprehensive inventory of the transcripts and proteins with altered abundances in response to a long photoperiod (LP) during growth stage transitions. We detected 22 000 transcripts in RNA-sequence runs and 5 259 proteins from an iTRAQ-based analysis. A weak correlation between mRNA- and protein-level changes was observed, suggesting the LP-induced transition between maize growth stages is largely regulated post-transcriptionally. Differentially expressed genes influenced by LP conditions were associated with several regulatory processes in both maize inbred lines, especially phosphate ion transport and the circadian rhythm. Additionally, 31 transcripts and six proteins related to photoperiodic flowering in maize were identified by comparing transcriptomic and proteomic data. This transcriptomic and proteomic analysis represents the first comprehensive and comparative study of gene/protein-level changes occurring in photoperiod-sensitive and -insensitive maize inbred lines during growth stage transitions under LP conditions.

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《农业科学学报（英文版）》 |2019年第6期|1211-1221|共11页
作者
SONG Xiao-heng; TIAN Lei; WANG Shun-xi; ZHOU Jin-Iong; ZHANG Jun; CHEN Zan; WU Liu-ji; KU Li-xia; CHEN Yan-hui;
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College of Agronomy, Henan Agricultural University/Synergetic Innovation Center of Henan Grain Crops/Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou 450002, P.R.China;

College of Agronomy, Henan Agricultural University/Synergetic Innovation Center of Henan Grain Crops/Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou 450002, P.R.China;

College of Agronomy, Henan Agricultural University/Synergetic Innovation Center of Henan Grain Crops/Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou 450002, P.R.China;

College of Agronomy, Henan Agricultural University/Synergetic Innovation Center of Henan Grain Crops/Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou 450002, P.R.China;

Henan Academy of Agricultural Science, Zhengzhou 450002, P.R.China;

College of Agronomy, Henan Agricultural University/Synergetic Innovation Center of Henan Grain Crops/Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou 450002, P.R.China;

College of Agronomy, Henan Agricultural University/Synergetic Innovation Center of Henan Grain Crops/Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou 450002, P.R.China;

College of Agronomy, Henan Agricultural University/Synergetic Innovation Center of Henan Grain Crops/Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou 450002, P.R.China;

College of Agronomy, Henan Agricultural University/Synergetic Innovation Center of Henan Grain Crops/Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou 450002, P.R.China;

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Integrating transcriptomic and proteomic analyses of photoperiod-sensitive in near isogenic maize line under long-day conditions

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