Weaning methods affect ruminal methanogenic archaea composition and diversity in Holstein calves

DONG Li-feng; MA Jun-nan; TU Yan; DIAO Qi-yu

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Weaning methods affect ruminal methanogenic archaea composition and diversity in Holstein calves

机译：断奶方法会影响荷斯坦牛犊的瘤胃产甲烷菌古菌组成和多样性

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The objective of the present study was to examine the effect of different weaning methods on the ruminal methanogenic archaea composition and diversity in Holstein calves.Thirty-six newborn Holstein bull calves were assigned to 1 of 3 treatments:(1) conventional weaning (d 56) and fed a high proportion of solid feed (CWS);(2) conventional weaning (d 56) and fed a high proportion of liquid feed (CWL);(3) early weaning (d 42) and fed with a high proportion of solid feed (EWS).High-throughput sequencing of the methyl coenzyme M reductase (mcrA) gene,which encodes the α-subunit of methyl coenzyme M reductase-the enzyme that catalyzes the final step in methanogenesis was used to determine the composition and diversity of rumen methanogens.No significant difference (P＞0.05) was observed for operational taxonomic units (OTUs) or richness indices,but diversity indices increased (P＜0.05) for calves fed high dietary solids.Predominant families across the three treatments were Methanobacteriaceae,Thermoplasmataceae and Methanomassiliicoccaceae.Calves in the EWS treatment had a higher (P＜0.05) relative abundance of Methanobrevibactersp.strain AbM4 and Methanosphaera stadtmanae,while calves in the CWL treatment had a higher (P＜0.05) abundance of Methanosphaera sp.strain SM9.A positive (P＜0.05) relationship was identified between butyrate and Methanobrevibactersp.strain AbM4.In conclusion,the composition and diversity of methanogens in the rumen of Holstein calves varied under the different weaning methods.This study identified a positive relationship between butyrate and Methanobrevibactersp.strain AbM4,potentially reflecting correlations between ruminal fermentation variables and methanogenesis function.These in-depth analyses provide further understanding of weaning methods for intensified production systems.

机译：本研究的目的是研究不同断奶方法对荷斯坦犊牛瘤胃产甲烷菌古菌组成和多样性的影响。将三十六种新生荷斯坦公牛犊分为3种处理方法之一：（1）常规断奶（d 56 ）并饲喂高比例的固体饲料（CWS）;（2）常规断奶（d 56）并饲喂高比例的液体饲料（CWL）;（3）早期断奶（d 42）并饲喂高比例的饲料固体辅料（EWS）。甲基辅酶M还原酶（mcrA）基因的高通量测序，该基因编码甲基辅酶M还原酶的α亚基-该酶催化产甲烷作用的最后一步用于确定组成和多样性饲喂高日粮固体的小牛的操作分类单位（OTUs）或丰富度指数无显着性差异（P＞ 0.05），但多样性指数增加（P ＜0.05）。三种处理的主要科系是甲烷细菌科EWS处理的犊牛的Methanobrevibactersp。菌株AbM4和St. Manthanosphaera stadtmanae的相对丰度较高（P ＜0.05），而CWL处理的犊牛的Methanosphaera sp.strain的相对丰度较高（P ＜0.05）。。丁酸与甲甲烷短杆菌属菌株AbM4之间存在正相关（P ＜0.05）。结论，不同断奶方式下荷斯坦犊牛瘤胃产甲烷菌的组成和多样性均存在差异。 Methanobrevibactersp。菌株AbM4，潜在地反映了瘤胃发酵变量和甲烷生成功能之间的相关性。这些深入的分析提供了对集约化生产系统的断奶方法的进一步理解。

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《农业科学学报（英文版）》 |2019年第5期|1080-1092|共13页
作者
DONG Li-feng; MA Jun-nan; TU Yan; DIAO Qi-yu;
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作者单位

Feed Research Institute, Chinese Academy of Agricultural Sciences/Beijing Key Laboratory for Dairy Cow Nutrition/Key Laboratory of Feed Biotechnology, Ministry of Agriculture, Beijing 100081, P.R.China;

Feed Research Institute, Chinese Academy of Agricultural Sciences/Beijing Key Laboratory for Dairy Cow Nutrition/Key Laboratory of Feed Biotechnology, Ministry of Agriculture, Beijing 100081, P.R.China;

Feed Research Institute, Chinese Academy of Agricultural Sciences/Beijing Key Laboratory for Dairy Cow Nutrition/Key Laboratory of Feed Biotechnology, Ministry of Agriculture, Beijing 100081, P.R.China;

Feed Research Institute, Chinese Academy of Agricultural Sciences/Beijing Key Laboratory for Dairy Cow Nutrition/Key Laboratory of Feed Biotechnology, Ministry of Agriculture, Beijing 100081, P.R.China;

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Weaning methods affect ruminal methanogenic archaea composition and diversity in Holstein calves

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