Molecular Cloning and Expression of Polyphenoloxidase Genes from the Mushroom,Agaricus bisporus

LI Nan-yi; CAI Wei-ming; JIN Qun-li; QIN Qiao-ping; RAN Fu-lai

首页> 外文期刊>农业科学学报（英文版） >Molecular Cloning and Expression of Polyphenoloxidase Genes from the Mushroom,Agaricus bisporus

【24h】

Molecular Cloning and Expression of Polyphenoloxidase Genes from the Mushroom,Agaricus bisporus

机译：蘑菇双孢蘑菇多酚氧化酶基因的分子克隆和表达

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

The polyphenoloxidase(PPO) is the key enzyme considered to be responsible for mushroom browning.By using homology cloning and rapid amplification of cDNA ends(RACE),two new PPO genes and the corresponding cDNA were identified from the fruit bodies of Agaricus bisporus(AbPPO3 and AbPPO4,GenBank accession nos.GU936494 and GU936493,respectively).The genomic DNA sequences of AbPPO3 and AbPPO4 are 2 080 and 2 189 bp in length,respectively,encoding putative polypeptides of approximately 66 and 68 kDa.The deduced amino acid sequences show characteristic features of two copper-binding domains conserved in the type III copper proteins including fungal polyphenol oxidases.Sequence comparisons indicate that AbPPO3 and AbPPO4 present 55.3% similarity to each other(48% identity).We also obtained more than 1.5-kb long sequences upstream of the start codon of the AbPPO3 and AbPPO4 and recognized them as their respective putative promoters.Analyses of the two PPO promoter regions show that they contain abundant cis-acting elements which are probably responsible for anaerobic induction,light,wound,stress,and auxin response.Semi-quantitative RT-PCR results indicate that AbPPO3 and AbPPO4 were highly expressed in the mature fruit bodies and up-regulated after 2-d storage of mushroom.These results suggest that AbPPO3 and AbPPO4 may play roles in A.bisporus browning and pigmentation during development and postharvest storage and the elements in promoters may act as regulatory elements for the inducible expression of AbPPO3 and AbPPO4.The successful cloning and expression analysis of AbPPO3 and AbPPO4 warrant a further investigation on the structure and function of A.bisporus PPO which points to the possible targets for genetic manipulation.

著录项

来源
《农业科学学报（英文版）》 |2011年第2期|185-194|共10页
作者
LI Nan-yi; CAI Wei-ming; JIN Qun-li; QIN Qiao-ping; RAN Fu-lai;
展开▼
作者单位

College of Agriculture and Food Science Zhejiang Agricultural and Forestry University Hangzhou 311300 P.R.China;

Institute of Horticulture Zhejiang Academy of Agricultural Sciences Hangzhou 310021 P.R.China;

Odessa College University of Ave Odessa 79764 USA;

展开▼
收录信息中国科学引文数据库(CSCD);
原文格式 PDF
正文语种 chi
中图分类
关键词

Molecular Cloning and Expression of Polyphenoloxidase Genes from the Mushroom,Agaricus bisporus

摘要

著录项

相关主题

期刊订阅